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Establishment of a congenital tooth agenesis related gene MSX1 knockout human embryonic stem cell lines by CRISPR-Cas9 technology
- Source :
- Stem Cell Research, Vol 24, Iss C, Pp 151-154 (2017)
- Publication Year :
- 2017
- Publisher :
- Elsevier, 2017.
-
Abstract
- Human MSX1 gene is mapped to chromosome 4 and encodes a 303aa homeobox protein MSX1. MSX1 expression appears during early tooth development of vertebrate embryogenesis. Mutations in this protein are related to human tooth anomalie, cleft lip and palate and congenital ectodermal dysplasia syndrome. Most of the confirmed pathogenic mutations are located in exon2 encoded homeobox domain. Here, we report the establishment of MSX1 gene knockout human embryonic stem (hES) cell lines by CRISPR-Cas9 technology. These cell lines provide good materials for further studies of the roles MSX1 plays in human tooth development and congenital tooth agenesis.
- Subjects :
- Male
0301 basic medicine
Human Embryonic Stem Cells
Homeobox A1
Biology
medicine.disease_cause
Cell Line
03 medical and health sciences
stomatognathic system
Human tooth development
medicine
Humans
Gene
lcsh:QH301-705.5
Gene knockout
MSX1 Transcription Factor
Genetics
Mutation
Cell Biology
General Medicine
Embryonic stem cell
stomatognathic diseases
030104 developmental biology
Chromosome 4
lcsh:Biology (General)
Odontogenesis
Homeobox
CRISPR-Cas Systems
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 18767753 and 18735061
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Stem Cell Research
- Accession number :
- edsair.doi.dedup.....21fb07049bcf71cf9c71057d1326699b