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Isolation of a rat amiloride-binding protein cDNA clone: tissue distribution and regulation of expression
- Source :
- The American journal of physiology. 266(6 Pt 1)
- Publication Year :
- 1994
-
Abstract
- Sodium transport across high-resistance epithelia involves both an apical amiloride-sensitive sodium channel and the basal Na(+)-K(+)-ATPase pump. Aldosterone regulates sodium transport by increasing the sodium permeability of the sodium channels. To study further the regulation of gene expression in sodium-transporting epithelia by corticosteroids, we have cloned an amiloride-binding protein (ABP) cDNA from rat descending colon and kidney. Identical 311 nucleotide cDNAs were amplified from both rat descending colon and kidney, and the predicted amino acid sequence exhibited 83% homology to the equivalent region of the human peptide sequence. Use of this cDNA as a probe resulted in detection of a transcript in both the small and large bowel, thymus, and seminal vesicle. The latter tissue exhibited the highest level of rat ABP expression. Low to undetectable levels of rat ABP were expressed in the descending colon and kidney. No regulation of rat ABP by either class of corticosteroids was observed. Levels of ABP were low at birth and increased gradually to adult levels just before weaning in the bowel. The distribution of rat ABP is not as would be predicted for an aldosterone-induced gene and is thus unlikely to be a component of the amiloride-sensitive electrogenic sodium channel.
- Subjects :
- Male
medicine.medical_specialty
Aging
DNA, Complementary
genetic structures
Physiology
Sodium
Molecular Sequence Data
chemistry.chemical_element
Gene Expression
Biology
Descending colon
RNA, Complementary
Rats, Sprague-Dawley
Adrenal Cortex Hormones
Ileum
Internal medicine
Complementary DNA
Gene expression
medicine
Animals
Humans
Tissue Distribution
Amino Acid Sequence
Cloning, Molecular
Peptide sequence
Regulation of gene expression
Base Sequence
Sodium channel
Nucleic Acid Hybridization
Cell Biology
Molecular biology
Amiloride
Rats
medicine.anatomical_structure
Endocrinology
chemistry
Molecular Probes
Amine Oxidase (Copper-Containing)
Carrier Proteins
medicine.drug
Subjects
Details
- ISSN :
- 00029513
- Volume :
- 266
- Issue :
- 6 Pt 1
- Database :
- OpenAIRE
- Journal :
- The American journal of physiology
- Accession number :
- edsair.doi.dedup.....21f34ce120db2bb59d95182e1a8dc28a