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Thymoquinone protects rat liver after partial hepatectomy under ischemia/reperfusion through oxidative stress and endoplasmic reticulum stress prevention
- Source :
- Clinical and Experimental Pharmacology and Physiology, Clinical and Experimental Pharmacology and Physiology, 2018, 〈10.1111/1440-1681.12961〉, Clinical and Experimental Pharmacology and Physiology, 2018, 45 (9), pp.943-951. ⟨10.1111/1440-1681.12961⟩, Clinical and Experimental Pharmacology and Physiology, Wiley, 2018, 45 (9), pp.943-951. ⟨10.1111/1440-1681.12961⟩
- Publication Year :
- 2018
- Publisher :
- HAL CCSD, 2018.
-
Abstract
- International audience; Ischemia reperfusion (I/R) is associated with liver injury and impaired regeneration during partial hepatectomy (PH). The aim of this study was to investigate the effect of thymoquinone (TQ), the active compound of essential oil obtained from Nigella sativa seeds, on rat liver after PH. Male Wistar rats were equally divided into four groups (n=6) receiving an oral administration of either vehicle solution (Sham and PH groups) or TQ at 30 mg/kg (TQ and TQ+PH groups) for ten consecutive days. Then, rats underwent PH (70%) with 60 min of ischemia followed by 24h of reperfusion (PH and TQ+PH groups). Alanine aminotransferase (ALT) activity and histopathological damage were determined. Also, antioxidant parameters, liver regeneration index, hepatic adenosine triphosphate (ATP) content, endoplasmic reticulum (ER) stress and apoptosis were assessed. In response to PH under I/R, liver damage was significantly alleviated by TQ treatment as evidenced by the decrease in ALT activity (P < 0.01) and histological findings (P < 0.001). In parallel, TQ preconditioning increased hepatic antioxidant capacities. Moreover, TQ improved mitochondrial function (ATP, P < 0.05), attenuated ER stress parameters and repressed the expression of apoptotic effectors. Taken together, our results suggest that TQ preconditioning could be an effective strategy to reduce liver injury after PH under I/R. The protective effects were mediated by the increase of antioxidant capacities and the decrease of ER stress and apoptosis. This article is protected by copyright. All rights reserved.
- Subjects :
- 0301 basic medicine
Physiology
medicine.medical_treatment
thymoquinone
Pharmacology
medicine.disease_cause
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
hepatectomy
Physiology (medical)
medicine
oxidative stress
Thymoquinone
Liver injury
[ SDV ] Life Sciences [q-bio]
Chemistry
Endoplasmic reticulum
apoptosis
[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
medicine.disease
Liver regeneration
[SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
030104 developmental biology
Apoptosis
030220 oncology & carcinogenesis
[SDV.SP.PHARMA] Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
Unfolded protein response
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
endoplasmic reticulum stress
Hepatectomy
Oxidative stress
Ischemia reperfusion injury
Subjects
Details
- Language :
- English
- ISSN :
- 14401681 and 03051870
- Database :
- OpenAIRE
- Journal :
- Clinical and Experimental Pharmacology and Physiology, Clinical and Experimental Pharmacology and Physiology, 2018, 〈10.1111/1440-1681.12961〉, Clinical and Experimental Pharmacology and Physiology, 2018, 45 (9), pp.943-951. ⟨10.1111/1440-1681.12961⟩, Clinical and Experimental Pharmacology and Physiology, Wiley, 2018, 45 (9), pp.943-951. ⟨10.1111/1440-1681.12961⟩
- Accession number :
- edsair.doi.dedup.....21dfd5d71de260aa82d4b2033f8ace9c