Back to Search
Start Over
Campylobacter jejuni Binds Intestinal H(O) Antigen (Fucα1, 2Galβ1, 4GlcNAc), and Fucosyloligosaccharides of Human Milk Inhibit Its Binding and Infection
- Source :
- Journal of Biological Chemistry. 278:14112-14120
- Publication Year :
- 2003
- Publisher :
- Elsevier BV, 2003.
-
Abstract
- The most common cause of infant mortality is diarrhea; the most common cause of bacterial diarrhea is Campylobacter jejuni, which is also the primary cause of motor neuron paralysis. The first step in campylobacter pathogenesis is adherence to intestinal mucosa. We found that such binding was inhibited in vitro by human milk and, with high avidity, by alpha1,2-fucosylated carbohydrate moieties containing the H(O) blood group epitope (Fuc alpha 1,2Gal beta 1,4GlcNAc em leader ). In studies on the mechanism of adherence, campylobacter, which normally does not bind to Chinese hamster ovary cells, bound avidly when the cells were transfected with a human alpha1,2-fucosyltransferase gene that caused overexpression of H-2 antigen; binding was specifically inhibited by H-2 ligands (lectins Ulex europaeus and Lotus tetragonolobus and H-2 monoclonal antibody), H-2 mimetics, and human milk oligosaccharides. Human milk oligosaccharides inhibited campylobacter colonization of mice in vivo and human intestinal mucosa ex vivo. Campylobacter colonization of nursing mouse pups was inhibited if their dams had been transfected with a human alpha1,2-fucosyltransferase gene that caused expression of H(O) antigen in milk. We conclude that campylobacter binding to intestinal H-2 antigen is essential for infection. Milk fucosyloligosaccharides and specific fucosyl alpha1,2-linked molecules inhibit this binding and may represent a novel class of antimicrobial agents.
- Subjects :
- Time Factors
Oligosaccharides
CHO Cells
Lotus tetragonolobus
Ligands
Transfection
medicine.disease_cause
Biochemistry
Campylobacter jejuni
Epitope
ABO Blood-Group System
Microbiology
Epitopes
Inhibitory Concentration 50
Mice
chemistry.chemical_compound
2'-Fucosyllactose
Antigen
Intestinal mucosa
Polysaccharides
Cricetinae
Lectins
Cell Adhesion
medicine
Animals
Humans
Antigens
Molecular Biology
Fucose
Neurons
Mice, Inbred BALB C
Dose-Response Relationship, Drug
Milk, Human
biology
Campylobacter
Chinese hamster ovary cell
O Antigens
Cell Biology
Fucosyltransferases
biology.organism_classification
Microscopy, Fluorescence
chemistry
Protein Binding
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 278
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....21acacb65cf06fde58c1aa9afaa71663