Second supplement for the paper chromatographic separation and identification of phenol derivatives and related compounds of biochemical interest, using a 'reference system'

Supplementary data are given for the paper-chromatographic investigation of mono-, di- and trihydric phenol derivatives and various compounds of biochemical interest, such as biogenic amines, aromatic and heterocyclic amino acids, hippuric acids, indole derivatives. Some further types of compounds such as alkaloids, hydroxylated quinoline and pyridine derivatives and antibiotics, not dealt with in previous investigations reported earlier in these Reviews, have now been included. The R F values of these compounds in six different solvent systems together with the corresponding colour reactions produced by ten standard reagents have been determined and are presented in 15 tables. Altogether approx. 270 compounds have been investigated and 20 hippuric acid derivatives synthetized. Application of these procedures to the clinical-chemical analysis of urine samples in order to detect metabolic excretion or detoxification products is indicated. The hippuric acid reagent was modified so that it contained p -dimethylamino-cinnamaldehyde in acetic acid anhydride. The Ehrlich reagent was prepared on the basis of p -dimethylaminocinnamaldehyde in hydrochloric acid; the colours produced with this reagent made it possible to differentiate the indole derivatives more distinctly. The relation between the chemical structure of the compounds studied and the R F values and colours produced with the detection reagents, is discussed.