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A FACS-optimized screen identifies regulators of genome stability in Candida albicans
- Source :
- Eukaryotic Cell, Eukaryotic Cell, American Society for Microbiology, 2015, 14 (3), pp.311-22. ⟨10.1128/EC.00286-14⟩, Eukaryotic Cell, 2015, 14 (3), pp.311-22. ⟨10.1128/EC.00286-14⟩
- Publication Year :
- 2015
- Publisher :
- HAL CCSD, 2015.
-
Abstract
- Loss of heterozygosity (LOH) plays important roles in genome dynamics, notably, during tumorigenesis. In the fungal pathogen Candida albicans , LOH contributes to the acquisition of antifungal resistance. In order to investigate the mechanisms that regulate LOH in C. albicans , we have established a novel method combining an artificial heterozygous locus harboring the blue fluorescent protein and green fluorescent protein markers and flow cytometry to detect LOH events at the single-cell level. Using this fluorescence-based method, we have confirmed that elevated temperature, treatment with methyl methanesulfonate, and inactivation of the Mec1 DNA damage checkpoint kinase triggered an increase in the frequency of LOH. Taking advantage of this system, we have searched for C. albicans genes whose overexpression triggered an increase in LOH and identified four candidates, some of which are known regulators of genome dynamics with human homologues contributing to cancer progression. Hence, the approach presented here will allow the implementation of new screens to identify genes that are important for genome stability in C. albicans and more generally in eukaryotic cells.
- Subjects :
- Genome instability
Locus (genetics)
Microbiology
Genomic Instability
Loss of heterozygosity
Fungal Proteins
Candida albicans
MESH: Cloning, Molecular
Cloning, Molecular
Molecular Biology
Gene
[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
Genetics
Fungal protein
biology
MESH: Candida albicans
MESH: Genomic Instability
General Medicine
Articles
G2-M DNA damage checkpoint
biology.organism_classification
Corpus albicans
MESH: Genome, Fungal
MESH: Fungal Proteins
Genome, Fungal
Subjects
Details
- Language :
- English
- ISSN :
- 15359778 and 15359786
- Database :
- OpenAIRE
- Journal :
- Eukaryotic Cell, Eukaryotic Cell, American Society for Microbiology, 2015, 14 (3), pp.311-22. ⟨10.1128/EC.00286-14⟩, Eukaryotic Cell, 2015, 14 (3), pp.311-22. ⟨10.1128/EC.00286-14⟩
- Accession number :
- edsair.doi.dedup.....219e3fdcf142a54ef9ac1b4281797256