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Effects of maternal feed intake restriction during pregnancy on the expression of growth regulation, imprinting and epigenetic transcription-related genes in foetal goats
- Source :
- Animal Reproduction Science. 198:90-98
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Maternal nutrition during gestation is a leading factor of modifying the foetal epigenome and phenotype for mammals. Imprinting genes have important roles in regulating foetal growth, programming and development. There, however, are limited data available on the effects of feed intake restriction on the expression of imprinting genes in pregnant goats. The present study, therefore, was conducted to assess the effects of maternal feed intake restriction on the relative abundance of mRNA for growth imprinting, DNA methyltransferase (DNMT) and epigenetic transcription-related genes in the liver and heart of foetal goats during gestation. A total of 24 Liuyang black goats (2.0±0.3 yr) with similar body weight (BW, 31.22±8.09 kg) and parity (2) were allocated equally to either a control group (CG) or a restriction group (RG) during both early (from 26 to 65 days) and late (from 96 to 135 days) gestation. All goats were fed a mixed diet and had free access to fresh water. The feed of the RG was 40% less than that of the CG. The early and late gestation goats were weighed, bled and slaughtered on days 65 and 135 of gestation, respectively. In early gestation, the foetal weight, body length, the weight of foetal heart and liver were greater (P 0.05) in the RG. The CpG methylation of genomic DNA in the foetal heart was less (P = 0.0001) in the RG. The relative abundance of mRNA of methyl-CpG-binding domain protein 2 (MBD2) and methyl-CpG-binding domain protein 3 (MBD3) genes in the foetal liver were greater (P 0.05) in the RG. During the late gestation, the foetal weight, heart weight and liver weight were less (P 0.05) in the RG. The relative abundance of mRNA for the MBD2 gene (P = 0.043) in the foetal heart, and the ten-eleven translocation protein 1 (TET1) gene (P 0.05) in both the foetal heart and liver were greater in the RG. These results indicate feed intake restriction during gestation influenced foetal development and regulated the relative abundance of mRNA for epigenetic transcription-related genes.
- Subjects :
- 0301 basic medicine
Biology
DNA methyltransferase
Epigenesis, Genetic
Fetal Development
Andrology
Genomic Imprinting
Random Allocation
03 medical and health sciences
Fetus
0302 clinical medicine
Endocrinology
Food Animals
Pregnancy
medicine
Animals
Epigenetics
Imprinting (psychology)
Caloric Restriction
Goats
Maternal Nutritional Physiological Phenomena
General Medicine
medicine.disease
Animal Feed
Phenotype
030104 developmental biology
DNA methylation
Gestation
Animal Nutritional Physiological Phenomena
Female
Animal Science and Zoology
Food Deprivation
Genomic imprinting
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 03784320
- Volume :
- 198
- Database :
- OpenAIRE
- Journal :
- Animal Reproduction Science
- Accession number :
- edsair.doi.dedup.....2198057c19d178450f98d182bbdca880
- Full Text :
- https://doi.org/10.1016/j.anireprosci.2018.09.005