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High dose folic acid supplementation of rats alters synaptic transmission and seizure susceptibility in offspring
- Source :
- Scientific Reports
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- Maternal folic acid supplementation is essential to reduce the risk of neural tube defects. We hypothesize that high levels of folic acid throughout gestation may produce neural networks more susceptible to seizure in offspring. We hence administered large doses of folic acid to rats before and during gestation and found their offspring had a 42% decrease in their seizure threshold. In vitro, acute application of folic acid or its metabolite 4Hfolate to neurons induced hyper-excitability and bursting. Cultured neuronal networks which develop in the presence of a low concentration (50 nM) of 4Hfolate had reduced capacity to stabilize their network dynamics after a burst of high-frequency activity, and an increase in the frequency of mEPSCs. Networks reared in the presence of the folic acid metabolite 5M4Hfolate developed a spontaneous, distinctive bursting pattern, and both metabolites produced an increase in synaptic density.
- Subjects :
- Male
medicine.medical_specialty
Offspring
Metabolite
Synaptophysin
Gestational Age
Biology
Neurotransmission
Synaptic Transmission
Article
Membrane Potentials
03 medical and health sciences
Bursting
chemistry.chemical_compound
Folic Acid
0302 clinical medicine
Pregnancy
Seizures
Internal medicine
medicine
Animals
Rats, Long-Evans
Cells, Cultured
030304 developmental biology
Neurons
0303 health sciences
Multidisciplinary
Dose-Response Relationship, Drug
Seizure threshold
Neural tube
Excitatory Postsynaptic Potentials
medicine.disease
Rats
Dose–response relationship
Endocrinology
medicine.anatomical_structure
chemistry
Dietary Supplements
Female
Nerve Net
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....218657fa715189e5b2847ab5d8028303