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Complexes from Trypanosoma brucei That Exhibit Deletion Editing and Other Editing-Associated Properties
- Source :
- Molecular and Cellular Biology. 16:1410-1418
- Publication Year :
- 1996
- Publisher :
- Informa UK Limited, 1996.
-
Abstract
- Transcripts from many mitochondrial genes in kinetoplastids undergo RNA editing, a posttranscriptional process which inserts and deletes uridines. By assaying for deletion editing in vitro, we found that the editing activity from Trypanosoma brucei mitochondrial lysates (S.D. Seiwert and K.D. Stuart), Science 266:114-117,1994) sediments with a peak of approximately 20S. RNA helicase, terminal uridylyl transferase, RNA ligase, and adenylation activities, which may have a role in editing, cosediment in a broad distribution, with most of each activity at 35 to 40S. Most ATPase 6 (A6) guide RNA and unedited A6 mRNA sediments at 20 to 30S, with some sedimenting further into the gradient, while most edited A6 mRNA sediments at >35S. Several mitochondrial proteins which cross-link specifically with guide RNA upon UV treatment also sediment in glycerol gradients. Notably, a 65-kDa protein sediments primarily at approximately 20S, a 90-kDa protein sediments at 35 to 40S, and a 25-kDa protein is present at
- Subjects :
- RNA, Mitochondrial
Molecular Sequence Data
Trypanosoma brucei brucei
Protozoan Proteins
Biology
Animals
Guide RNA
Molecular Biology
Sequence Deletion
Ribonucleoprotein
RNA ligase
Messenger RNA
Base Sequence
Intron
RNA
Cell Biology
Blotting, Northern
RNA Helicase A
Molecular biology
Mitochondria
Biochemistry
RNA editing
RNA Editing
Oligonucleotide Probes
RNA, Protozoan
Research Article
Subjects
Details
- ISSN :
- 10985549
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Molecular and Cellular Biology
- Accession number :
- edsair.doi.dedup.....217119430972999a9a900b684481d87d