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Hos1 deacetylates Smc3 to close the cohesin acetylation cycle
- Source :
- Molecular cell. 39(5)
- Publication Year :
- 2010
-
Abstract
- Cohesion between sister chromatids is mediated by the chromosomal cohesin complex. In budding yeast, cohesin is loaded onto chromosomes during the G1 phase of the cell cycle. During S phase, the replication fork-associated acetyltransferase Eco1 acetylates the cohesin subunit Smc3 to promote the establishment of sister chromatid cohesion. At the time of anaphase, Smc3 loses its acetylation again, but the Smc3 deacetylase and the possible importance of Smc3 deacetylation are unknown. Here, we show that the class I histone deacetylase family member Hos1 is responsible for Smc3 deacetylation. Cohesin is protected from deacetylation while bound to chromosomes but is deacetylated as soon as it dissociates from chromosomes at anaphase onset. Nonacetylated Smc3 is required as a substrate for cohesion establishment in the following cell cycle. Our results complete the description of an Smc3 acetylation cycle and provide unexpected insight into the importance of de novo Smc3 acetylation for cohesion establishment.
- Subjects :
- Saccharomyces cerevisiae Proteins
Cohesin complex
Chromosomal Proteins, Non-Histone
Cell Cycle Proteins
Saccharomyces cerevisiae
Biology
Histone Deacetylases
S Phase
Acetyltransferases
Sister chromatids
Molecular Biology
Anaphase
Genetics
Cohesin
G1 Phase
Nuclear Proteins
Acetylation
Cell Biology
Cell biology
Establishment of sister chromatid cohesion
Histone deacetylase
biological phenomena, cell phenomena, and immunity
Separase
Chromosomes, Fungal
Subjects
Details
- ISSN :
- 10974164
- Volume :
- 39
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Molecular cell
- Accession number :
- edsair.doi.dedup.....21704564aabba99bb24c4cadb04f39d5