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A ‘tool box’ for deciphering neuronal circuits in the developing chick spinal cord
- Source :
- Nucleic Acids Research
- Publication Year :
- 2014
- Publisher :
- Oxford University Press (OUP), 2014.
-
Abstract
- The genetic dissection of spinal circuits is an essential new means for understanding the neural basis of mammalian behavior. Molecular targeting of specific neuronal populations, a key instrument in the genetic dissection of neuronal circuits in the mouse model, is a complex and time-demanding process. Here we present a circuit-deciphering ‘tool box’ for fast, reliable and cheap genetic targeting of neuronal circuits in the developing spinal cord of the chick. We demonstrate targeting of motoneurons and spinal interneurons, mapping of axonal trajectories and synaptic targeting in both single and populations of spinal interneurons, and viral vector-mediated labeling of pre-motoneurons. We also demonstrate fluorescent imaging of the activity pattern of defined spinal neurons during rhythmic motor behavior, and assess the role of channel rhodopsin-targeted population of interneurons in rhythmic behavior using specific photoactivation.
- Subjects :
- Rhodopsin
Nerve net
Population
Chick Embryo
Biology
Fluorescent imaging
Molecular targeting
Genes, Reporter
Interneurons
Connectome
Genetics
medicine
Animals
education
Motor Neurons
Genetic dissection
education.field_of_study
Integrases
Anatomy
Spinal cord
Axons
Enhancer Elements, Genetic
medicine.anatomical_structure
Spinal Cord
nervous system
Neuronal circuits
Synapses
Methods Online
Calcium
Nerve Net
Neuroscience
Subjects
Details
- ISSN :
- 13624962 and 03051048
- Volume :
- 42
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....2152146eac7174f29b280e59f9dd5447