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Comparison of a VLP‐based and GST‐L1‐based multiplex immunoassay to detect vaccine‐induced HPV‐specific antibodies in first‐void urine
- Source :
- Article, Journal of medical virology, Journal of Medical Virology
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Vaccine‐induced human papillomavirus (HPV) antibodies originating from cervicovaginal secretions were recently shown to be detectable in first‐void (FV) urine. This presents a novel opportunity for noninvasive sampling to monitor HPV antibody status in women participating in large epidemiological studies and HPV vaccine trials. With a view towards method optimization, this study compared the measurement of HPV antibodies in FV urine using a multiplex L1/L2 virus‐like particles (VLP)‐based ELISA (M4ELISA) with previously reported results using a glutathione S‐transferase (GST)‐L1‐based immunoassay (GST‐L1‐MIA). We tested 53 paired FV urine and serum samples from 19‐ to 26‐year‐old healthy women, unvaccinated (n = 17) or vaccinated with either the bivalent or quadrivalent HPV‐vaccine during adolescence (n = 36). HPV6/11/16/18 antibodies were measured using M4ELISA and compared with GST‐L1‐MIA results. Inter‐assay and inter‐specimen correlations were examined using the Spearman's rank test (rs). As expected, lower HPV antibody concentrations were found in FV urine than in serum. Vaccinated women had significantly higher HPV6/11/16/18 antibody levels in both FV urine and serum compared with those unvaccinated (M4ELISA; FV urine P = .0003; serum P ≤ .0001). HPV antibody levels in FV urine and serum showed a significant positive correlation (M4ELISA anti‐HPV6/11/16/18, r s = 0.85/0.86/0.91/0.79, P ≤ .001). Despite assay differences, there was moderate to good correlation between M4ELISA and GST‐L1‐MIA (FV urine anti‐HPV6/11/16/18, r s = 0.86/0.83/0.89/0.53, P ≤ .0001; serum anti‐HPV6/11/16/18, r s = 0.93/0.89/0.94/0.75, P ≤ .0001). FV urine HPV antibody detection is comparable with both assays, further supporting this noninvasive sampling method as a possible option for HPV vaccine assessment. Approaches to improve the sensitivity and larger studies are warranted to determine the feasibility of FV urine for vaccine‐induced HPV antibody detection.<br />Highlights First‐void urine HPV antibody detection is comparable with both assays.Urine of vaccinees contain higher antibody levels as opposed to unvaccinated women.Urinary HPV6/11/16/18 antibodies correlate significantly with paired sera.Urine might offer a non‐invasive tool to assess serum antibody transudate.Approaches to improve the sensitivity of vaccine‐induced HPV antibody detection in FV urine are required.
- Subjects :
- Urine
HPV vaccines
HPV serology
03 medical and health sciences
0302 clinical medicine
First void urine
Virology
Medicine
Multiplex
030212 general & internal medicine
Human papillomavirus
HPV antibodies
human papillomavirus
Research Articles
biology
medicine.diagnostic_test
business.industry
urine
Specific antibody
Infectious Diseases
Immunoassay
biology.protein
030211 gastroenterology & hepatology
Human medicine
Antibody
business
Research Article
Subjects
Details
- ISSN :
- 10969071 and 01466615
- Volume :
- 92
- Database :
- OpenAIRE
- Journal :
- Journal of Medical Virology
- Accession number :
- edsair.doi.dedup.....213a5ec24741953b8e856e0692d6c0ec