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Overcoming the biological aging of titanium using a wet storage method after ultraviolet treatment
- Source :
- Scientific Reports, Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017), SCIENTIFIC REPORTS(7)
- Publication Year :
- 2017
-
Abstract
- We evaluated whether the biological activity of the surface of titanium, when stored in an aqueous solution after ultraviolet (UV) treatment, is comparable to that of the surface immediately after UV treatment. We subjected Grade IV titanium discs with machined surfaces to UV radiation for 15 min and then tested them immediately and after storage for 28 days, with and without distilled H2O (dH2O). We evaluated the surface characteristics using surface profiling, contact angle analysis, X-ray photoelectron spectroscopy, and in terms of the surface zeta-potential. We determined the level of biological activity by analysing albumin adsorption, MC3T3-E1 and human mesenchymal cell adhesion and cytoskeleton development, as well as the production of intracellular reactive oxygen species between groups. The surface characteristics produced by the UV irradiation were maintained in dH2O for 28 days. We found that titanium stored in dH2O for 28 days after UV treatment exhibited enhanced protein adsorption, cell attachment, and cytoskeleton development. Titanium stored in dH2O for 28 days after UV irradiation exhibited a lower level of oxidative stress, comparable to that of the titanium immediately after UV treatment. UV treatment combined with wet storage can be used as a means of overcoming the biological aging of titanium.
- Subjects :
- Surface Properties
Ultraviolet Rays
Science
Cell Culture Techniques
chemistry.chemical_element
02 engineering and technology
medicine.disease_cause
Article
Cell Line
Contact angle
03 medical and health sciences
Mice
0302 clinical medicine
Adsorption
X-ray photoelectron spectroscopy
Albumins
medicine
Cell Adhesion
Animals
Humans
Irradiation
Titanium
Multidisciplinary
Aqueous solution
030206 dentistry
021001 nanoscience & nanotechnology
chemistry
Medicine
0210 nano-technology
Reactive Oxygen Species
Ultraviolet
Nuclear chemistry
Protein adsorption
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....2117ae898d0b9335b9122cd439aa276b