Back to Search
Start Over
Identification of Key Genes Associated with Colorectal Cancer Based on the Transcriptional Network
- Source :
- Pathology & Oncology Research. 21:719-725
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- Colorectal cancer (CRC) is among the most lethal human cancers, but the mechanism of the cancer is still unclear enough. We aimed to explore the key genes in CRC progression. The gene expression profile (GSE4183) of CRC was obtained from Gene Expression Omnibus database which included 8 normal samples, 15 adenoma samples, 15 CRC samples and 15 inflammatory bowel disease (IBD) samples. Thereinto, 8 normal, 15 adenoma, and 15 CRC samples were chosen for our research. The differentially expressed genes (DEGs) in normal vs. adenoma, normal vs. CRC, and adenoma vs. CRC, were identified using the Wilcoxon test method in R respectively. The interactive network of DEGs was constructed to select the significant modules using the Pearson's correlation. Meanwhile, transcriptional network of DEGs was also constructed using the g: Profiler. Totally, 2,741 DEGs in normal vs. adenoma, 1,484 DEGs in normal vs. CRC, and 396 DEGs in adenoma vs. CRC were identified. Moreover, function analysis of DEGs in each group showed FcR-mediated phagocytosis pathway in module 1, cardiac muscle contraction pathway in module 6, and Jak-STAT signaling pathway in module 19 were also enriched. Furthermore, MZF1 and AP2 were the transcription factor in module 6, with the target SP1, while SP1 was also a transcription in module 20. DEGs like NCF1, AKT, SP1, AP2, MZF1, and TPM might be used as specific biomarkers in CRC development. Therapy targeting on the functions of these key genes might provide novel perspective for CRC treatment.
- Subjects :
- Adenoma
Cancer Research
Colon
Colorectal cancer
Gene regulatory network
Biology
Bioinformatics
Pathology and Forensic Medicine
Gene expression
Biomarkers, Tumor
medicine
Humans
Gene Regulatory Networks
Protein kinase B
Transcription factor
Regulation of gene expression
Gene Expression Profiling
Rectum
Computational Biology
General Medicine
medicine.disease
digestive system diseases
Gene Expression Regulation, Neoplastic
Gene expression profiling
Oncology
Cancer research
Colorectal Neoplasms
Transcription Factors
Subjects
Details
- ISSN :
- 15322807 and 12194956
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Pathology & Oncology Research
- Accession number :
- edsair.doi.dedup.....20fbeca1e04717a48d321f74745c7284