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Effects ofActinobacillus pleuropneumoniaeon barrier function and inflammatory response of pig tracheal epithelial cells
- Source :
- Pathogens and Disease. 77
- Publication Year :
- 2018
- Publisher :
- Oxford University Press (OUP), 2018.
-
Abstract
- Actinobacillus pleuropneumoniae is a respiratory pathogen that causes porcine pleuropneumonia, a fatal respiratory disease responsible for high economic losses in the swine industry worldwide. With the objective to better understand the interactions between A. pleuropneumoniae and the porcine respiratory epithelium, we investigated the capacity of this pathogen to damage the epithelial barrier and induce an inflammatory response. We showed that A. pleuropneumoniae, even at a multiplicity of infection of 10, is able to break the tracheal epithelial barrier integrity as determined by monitoring the transepithelial electrical resistance and fluorescein-isothiocyanate-dextran transport. Immunofluorescence staining analysis suggested that A. pleuropneumoniae is affecting two important tight junction proteins (occludin, zonula occludens-1). As a consequence of the breakdown of the epithelial barrier integrity, A. pleuropneumoniae can translocate across a cell monolayer. We also showed that tracheal epithelial cells secrete pro-inflammatory cytokines (IL-8, IL-6, TNF-α) in response to a stimulation with this pathogen. In summary, A. pleuropneumoniae is able to induce damage to the porcine respiratory epithelial barrier. Challenging the epithelial cells with A. pleuropneumoniae was also associated with the secretion of pro-inflammatory cytokines. This better knowledge of the interactions between A. pleuropneumoniae and the epithelial cells may help to design novel strategies to prevent epithelium invasion by this bacterium along with other swine respiratory pathogens.
- Subjects :
- Microbiology (medical)
Cell Survival
Swine
animal diseases
Respiratory Mucosa
Occludin
Tight Junctions
Microbiology
03 medical and health sciences
Actinobacillus Infections
Multiplicity of infection
medicine
Animals
Immunology and Allergy
Pathogen
Actinobacillus pleuropneumoniae
Barrier function
030304 developmental biology
Swine Diseases
0303 health sciences
General Immunology and Microbiology
biology
Tight junction
030306 microbiology
Epithelial Cells
General Medicine
respiratory system
bacterial infections and mycoses
biology.organism_classification
Epithelium
respiratory tract diseases
3. Good health
Infectious Diseases
medicine.anatomical_structure
Cytokines
Respiratory epithelium
Inflammation Mediators
Biomarkers
Subjects
Details
- ISSN :
- 2049632X
- Volume :
- 77
- Database :
- OpenAIRE
- Journal :
- Pathogens and Disease
- Accession number :
- edsair.doi.dedup.....20efdb61f77122c40350d91e1df2081e