Ratiometric activatable cell-penetrating peptides provide rapid in vivo readout of thrombin activation

Authors :: Quyen T. Nguyen
Nadia Nashi
Roger Y. Tsien
Michael A. Whitney
Stephen R. Adams
Rachel A. Levin
Heather L. Glasgow
Elamprakash N. Savariar
Roy B. Lefkowitz
Beth Friedman
Jessica L. Crisp
Paul Steinbach
Source :: Angewandte Chemie (International ed. in English), vol 52, iss 1, Whitney, M; Savariar, EN; Friedman, B; Levin, RA; Crisp, JL; Glasgow, HL; et al.(2013). Ratiometric activatable cell-penetrating peptides provide rapid in vivo readout of thrombin activation. Angewandte Chemie-International Edition, 52(1), 325-330. doi: 10.1002/anie.201205721. UC San Diego: Retrieved from: http://www.escholarship.org/uc/item/1pg2r418, Angewandte Chemie (International Ed. in English)
Publication Year :: 2013
Publisher :: eScholarship, University of California, 2013.
Abstract: In real time: Thrombin activation in vivo can be imaged in real time with ratiometric activatable cell penetrating peptides (RACPPs). RACPPs are designed to combine 1) dual-emission ratioing, 2) far red to infrared wavelengths for in vivo mammalian imaging, and 3) cleavage-dependent spatial localization. The most advanced RACPP uses norleucine (Nle)-TPRSFL as a linker that increases sensitivity to thrombin by about 90-fold (see figure). © 2013 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Database :: OpenAIRE
Journal :: Angewandte Chemie (International ed. in English), vol 52, iss 1, Whitney, M; Savariar, EN; Friedman, B; Levin, RA; Crisp, JL; Glasgow, HL; et al.(2013). Ratiometric activatable cell-penetrating peptides provide rapid in vivo readout of thrombin activation. Angewandte Chemie-International Edition, 52(1), 325-330. doi: 10.1002/anie.201205721. UC San Diego: Retrieved from: http://www.escholarship.org/uc/item/1pg2r418, Angewandte Chemie (International Ed. in English)
Accession number :: edsair.doi.dedup.....20dbfa6c6e9a314e2cfe2532ad43dc15

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