Additional file 5 of A comprehensive metagenomics framework to characterize organisms relevant for planetary protection

Additional file 4: Figure S3. Comparison of QC Metrics in cases to controls. We detected 24 species across all control samples after initial filtering. Environmental metagenomic samples are qualitatively similar to samples of laboratory contamination so filtering based on controls is non-trivial. We compared the number of unique marker k-mers found for each taxa in each case sample to the maximum number found in one control (A) and, analogously, the total number of reads (B). We took all taxonomic assignments with a read ratio greater than 2 and a k-mer ratio greater than 5 (C). If a taxonomic assignment met both criteria in at least two samples, it was permitted (red taxa on bottom right) otherwise the taxa was filtered from all assignments (blue taxa on bottom right).