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Proteomic Identification of Serum Proteins Associated with Stress-Induced Gastric Ulcers in Fasted Rats
- Source :
- Bioscience, Biotechnology, and Biochemistry. 74:812-818
- Publication Year :
- 2010
- Publisher :
- Oxford University Press (OUP), 2010.
-
Abstract
- Several physical and psychological stresses frequently become triggers for gastrointestinal disorders such as ulcer. In this study, we tried to identify serum proteins as potential biomarkers for the evaluation of stress-induced gastric ulcer. By proteomic analysis using rats with gastric ulcer induced by water immersion and restraint (WIR) stress as an animal model, we found quantitative changes in several serum proteins, including creatine kinase muscle M chain (CK-M) and apolipoprotein A-IV (ApoA4) in the stressed rats. On western blotting and enzyme-linked immunosorbent assay (ELISA), we confirmed that serum CK-M was remarkably increased by WIR stress. However, ApoA4 appeared to be decreased by fasting, but not WIR stress, which is usually applied prior to WIR stress. The findings suggest that these two serum proteins might be useful as biomarkers, CK-M for stress-induced gastric ulcer and ApoA4 for starvation.
- Subjects :
- Male
medicine.medical_specialty
Apolipoprotein B
Enzyme-Linked Immunosorbent Assay
Apolipoprotein A-IV
Proteomics
Applied Microbiology and Biotechnology
Biochemistry
Analytical Chemistry
Rats, Sprague-Dawley
APOA4
Internal medicine
Immersion
Animals
Medicine
Stomach Ulcer
Molecular Biology
Apolipoproteins A
chemistry.chemical_classification
biology
business.industry
Organic Chemistry
Proteins
Blood Proteins
General Medicine
Blood proteins
digestive system diseases
Rats
Blot
Enzyme
Endocrinology
chemistry
Duodenal Ulcer
biology.protein
Creatine kinase
business
Stress, Psychological
Biotechnology
Subjects
Details
- ISSN :
- 13476947 and 09168451
- Volume :
- 74
- Database :
- OpenAIRE
- Journal :
- Bioscience, Biotechnology, and Biochemistry
- Accession number :
- edsair.doi.dedup.....20d175f23e7a1e712e57f55169c0031c