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Electrochemical Biosensor for Nitrite Based on Polyacrylic-Graphene Composite Film with Covalently Immobilized Hemoglobin
- Source :
- Sensors (Basel, Switzerland), Sensors; Volume 18; Issue 5; Pages: 1343, Sensors, Vol 18, Iss 5, p 1343 (2018)
- Publication Year :
- 2018
- Publisher :
- MDPI, 2018.
-
Abstract
- A new biosensor for the analysis of nitrite in food was developed based on hemoglobin (Hb) covalently immobilized on the succinimide functionalized poly(n-butyl acrylate)-graphene [poly(nBA)-rGO] composite film deposited on a carbon-paste screen-printed electrode (SPE). The immobilized Hb on the poly(nBA)-rGO conducting matrix exhibited electrocatalytic ability for the reduction of nitrite with significant enhancement in the reduction peak at −0.6 V versus Ag/AgCl reference electrode. Thus, direct determination of nitrite can be achieved by monitoring the cathodic peak current signal of the proposed polyacrylic-graphene hybrid film-based voltammetric nitrite biosensor. The nitrite biosensor exhibited a reproducible dynamic linear response range from 0.05–5 mg L−1 nitrite and a detection limit of 0.03 mg L−1. No significant interference was observed by potential interfering ions such as Ca2+, Na+, K+, NH4+, Mg2+, and NO3− ions. Analysis of nitrite in both raw and processed edible bird’s nest (EBN) samples demonstrated recovery of close to 100%. The covalent immobilization of Hb on poly(nBA)-rGO composite film has improved the performance of the electrochemical nitrite biosensor in terms of broader detection range, lower detection limit, and prolonged biosensor stability.
- Subjects :
- 02 engineering and technology
Biosensing Techniques
Electrochemistry
lcsh:Chemical technology
01 natural sciences
Biochemistry
Reference electrode
Article
Analytical Chemistry
Matrix (chemical analysis)
chemistry.chemical_compound
Hemoglobins
Succinimide
lcsh:TP1-1185
Electrical and Electronic Engineering
Nitrite
nitrite
Instrumentation
Electrodes
Nitrites
edible bird’s nest
Detection limit
electrochemical biosensor
graphene
hemoglobin
010401 analytical chemistry
Electrochemical Techniques
021001 nanoscience & nanotechnology
Enzymes, Immobilized
Atomic and Molecular Physics, and Optics
0104 chemical sciences
chemistry
Electrode
Graphite
0210 nano-technology
Biosensor
Nuclear chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 14248220
- Volume :
- 18
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Sensors (Basel, Switzerland)
- Accession number :
- edsair.doi.dedup.....20cae367b9997f0af9cae83aea7dded2