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Immunogenicity of a Vaccine Preparation Representing the Variable Regions of the HIV Type 1 Envelope Glycoprotein
- Source :
- AIDS Research and Human Retroviruses. 16:153-161
- Publication Year :
- 2000
- Publisher :
- Mary Ann Liebert Inc, 2000.
-
Abstract
- Variability of the major antigenic sites of the envelope glycoprotein of HIV-1 constitutes a major problem in the formulation of effective vaccines. We have prepared a synthetic peptide vaccine that represents the major hypervariable epitopes (V1 through V5) of the clade B HIV-1 envelope glycoprotein (gp120). We refer to this preparation as variable epitope immunogen or VEI vaccine. This construct takes into consideration the type and frequency of amino acid substitutions found at each epitope during the evolution of the virus in individual patients and in the target population. Immunization of mice, rabbits, and rhesus macaques with the VEI vaccine resulted in the induction of long-lasting, high-titered HIV-1 antibodies, including antibodies that neutralize primary isolates. We also documented lymphocyte proliferative responses to the VEI vaccine, its individual components, analogs, and subtype-specific peptides representing the major hypervariable regions of HIV-1 gp120. Delayed-type hypersensitivity responses to these antigens were also demonstrated in mice. Our results show that this vaccine is highly immunogenic and safe in animals. Our data suggest that this formulation could become an important component of combination vaccine approaches against HIV-1 and other antigenically variable pathogens.
- Subjects :
- Cellular immunity
Immunogen
Molecular Sequence Data
Immunology
HIV Infections
HIV Envelope Protein gp120
Biology
Epitope
Mice
Antigen
Viral envelope
Virology
Animals
Amino Acid Sequence
AIDS Vaccines
Mice, Inbred BALB C
Mice, Inbred ICR
Vaccines, Synthetic
Immunogenicity
Macaca mulatta
Peptide Fragments
Hypervariable region
Infectious Diseases
HIV-1
Peptide vaccine
Rabbits
Sequence Alignment
Subjects
Details
- ISSN :
- 19318405 and 08892229
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- AIDS Research and Human Retroviruses
- Accession number :
- edsair.doi.dedup.....20c88ecce0b16bd8e17e260d82002c06