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Automated Patch Clamp Meets High-Throughput Screening: 384 Cells Recorded in Parallel on a Planar Patch Clamp Module

Authors :
Claudia Haarmann
Max Mueller
Juergen Steindl
Alison Obergrussberger
Andrea Brüggemann
Johannes Stiehler
Atsushi Ohtsuki
Niels Fertig
Nadine Becker
Marius Vogel
Tom A. Goetze
Ilka Rinke
Michael George
Takayuki Oka
Timo Stengel
Markus Rapedius
Source :
SLAS Technology. 21:779-793
Publication Year :
2016
Publisher :
Elsevier BV, 2016.

Abstract

We have developed an automated patch clamp module for high-throughput ion channel screening, recording from 384 cells simultaneously. The module is incorporated into a laboratory pipetting robot and uses a 384-channel pipettor head for application of cells and compounds. The module contains 384 amplifier channels for fully parallel recordings using a digital amplifier. Success rates for completed experiments (1- to 4-point concentration-response curves for cells satisfying defined quality control parameters) of greater than 85% have been routinely achieved with, for example, HEK, CHO, and RBL cell lines expressing hNaV1.7, hERG, Kir2.1, GABA, or glutamate receptors. Pharmacology experiments are recorded and analyzed using specialized software, and the pharmacology of hNaV1.7 and hERG is described. Fast external solution exchange rates of 200 MΩ, and recordings of voltage-gated Na+ and Ca2+ are shown.

Details

ISSN :
24726303
Volume :
21
Database :
OpenAIRE
Journal :
SLAS Technology
Accession number :
edsair.doi.dedup.....20c31bb0ac574bd86f5dc089b001c3ac