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Proteomic profiling of human respiratory epithelia by iTRAQ reveals biomarkers of exposure and harm by tobacco smoke components
- Source :
- Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals. 16(7)
- Publication Year :
- 2011
-
Abstract
- Historically, it has been challenging to go beyond epidemiology to investigate the pathogenic changes caused by tobacco smoking. The EpiAirway-100 (MatTek Corp., Ashland, MA) was employed to investigate the effects of cigarette smoke components. Exposure at the air-liquid-interface represented particle and vapour phase components of cigarette smoke. A proteomic study utilising iTRAQ labelling compared expression profiles. The correlative histopathology revealed focal regions of hyperplasia, hypertrophy, cytolysis and necrosis. We identified 466 proteins, 250 with a parameter of two or more peptides. Four of these proteins are potential markers of lung injury and three are related to mechanistic pathways of disease.
- Subjects :
- Proteomics
Proteome
Proteomic Profiling
Chemistry
Health, Toxicology and Mutagenesis
Gene Expression Profiling
Clinical Biochemistry
Blotting, Western
Smoking
Respiratory Mucosa
Lung injury
Biochemistry
Molecular biology
Tobacco smoke
Blot
Gene expression profiling
Cytolysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Immunology
Humans
Electrophoresis, Polyacrylamide Gel
Biomarkers
Cells, Cultured
Subjects
Details
- ISSN :
- 13665804
- Volume :
- 16
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals
- Accession number :
- edsair.doi.dedup.....209902ab1d488c3433d2aabe99e4de99