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MicroRNA Expression Profiles Associated with Mutational Status and Survival in Malignant Melanoma
- Source :
- Journal of Investigative Dermatology. 130:2062-2070
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- Malignant cutaneous melanoma is a highly aggressive form of skin cancer. Despite improvements in early melanoma diagnosis, the 5-year survival rate remains low in advanced disease. Therefore, novel biomarkers are urgently needed to devise new means of detection and treatment. In this study, we aimed to improve our understanding of microRNA (miRNA) deregulation in melanoma development and their impact on patient survival. Global miRNA expression profiles of a set of melanoma lymph node metastases, melanoma cell lines, and melanocyte cultures were determined using Agilent array. Deregulated miRNAs were evaluated in relation with clinical characteristics, patient survival, and mutational status for BRAF and NRAS. Several miRNAs were differentially expressed between melanocytes and melanomas as well as melanoma cell lines. In melanomas, miR-193a, miR-338, and miR-565 were underexpressed in cases with a BRAF mutation. Furthermore, low expression of miR-191 and high expression of miR-193b were associated with poor melanoma-specific survival. In conclusion, our findings show miRNA dysregulation in malignant melanoma and its relation to established molecular backgrounds of BRAF and NRAS oncogenic mutations. The identification of an miRNA classifier for poor survival may lead to the development of miRNA detection as a complementary prognostic tool in clinical practice.
- Subjects :
- Adult
Male
Proto-Oncogene Proteins B-raf
Neuroblastoma RAS viral oncogene homolog
Skin Neoplasms
Adolescent
DNA Mutational Analysis
Dermatology
Biochemistry
Young Adult
microRNA
Humans
Medicine
Melanoma
neoplasms
Molecular Biology
Survival rate
Aged
Aged, 80 and over
Regulation of gene expression
business.industry
Gene Expression Profiling
Cancer
Cell Biology
Middle Aged
Prognosis
medicine.disease
Gene Expression Regulation, Neoplastic
Gene expression profiling
MicroRNAs
Genes, ras
Lymphatic Metastasis
Immunology
Cancer research
Melanocytes
Female
Skin cancer
business
Genome-Wide Association Study
Subjects
Details
- ISSN :
- 0022202X
- Volume :
- 130
- Database :
- OpenAIRE
- Journal :
- Journal of Investigative Dermatology
- Accession number :
- edsair.doi.dedup.....2097665793e821eb8d334420f3a46599