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Is detection of intraperitoneal exfoliated tumor cells after surgical resection of rectal cancer a prognostic factor of survival?
- Source :
- BMC Cancer, Vol 17, Iss 1, Pp 1-8 (2017), BMC Cancer
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Background The prognostic significance of free cancer cells detected in peritoneal fluid at the time of rectal surgery remains unclear. A substantial number of patients will develop metastatic disease even with successful local treatment. This prospective non-randomized study investigated the prognostic value of intraperitoneal free cancer cells harvested in peritoneal lavage after surgery for rectal cancer. Mutational hotspots in mitochondrial DNA were examined as potential molecular signatures to detect circulating intraperitoneal free cancer cells when present in primary tumor and in lavage. Methods Point mutations in mitochondrial DNA amplifications were determined in primary tumors and corresponding exfoliated intraperitoneal free cancer cells in lavage from 191 patients with locally advanced rectal cancer scheduled for radical treatment. Mitochondrial DNA target sequences were amplified by polymerase chain reaction and base substitutions were detected by denaturant, cycling temperature capillary electrophoresis. Detection of intraperitoneal free cancer cells was correlated to survival. Results Of 191patients analyzed, 138 (72%) were identified with somatic mitochondrial point mutations in rectal cancer tumors. From this fraction, 45 patients (33%) had positive lavage fluid with corresponding somatic mtDNA point mutations in lavage representing circulating intraperitoneal free cancer cells. There was no significant survival difference between patients identified with or without somatic mitochondrial DNA point mutations in the corresponding lavage. Conclusion Somatic mitochondrial DNA point mutations identified in primary rectal tumors enable detection of circulating intraperitoneal free cancer cells in lavage fluid. Intraperitoneal free cancer cells harvested from lavage immediately after surgery for rectal cancer does not represent an independent prognostic factor on survival.
- Subjects :
- Adult
Male
Cancer Research
Pathology
medicine.medical_specialty
Survival
Somatic cell
Colorectal cancer
030230 surgery
lcsh:RC254-282
White People
03 medical and health sciences
mtDNA mutations
0302 clinical medicine
Surgical oncology
Genetics
Ascitic Fluid
Humans
Medicine
Peritoneal Lavage
Prospective Studies
Rectal cancer
Digestive System Surgical Procedures
Aged
Aged, 80 and over
Prognostic factor
Norway
Rectal Neoplasms
business.industry
Peritoneal fluid
Point mutation
Cancer
Middle Aged
Neoplastic Cells, Circulating
Prognosis
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
medicine.disease
Primary tumor
Oncology
030220 oncology & carcinogenesis
Cancer cell
Female
business
Research Article
Subjects
Details
- ISSN :
- 14712407
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- BMC Cancer
- Accession number :
- edsair.doi.dedup.....2096b7901d05e72ad3a68bebb25e7146