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Preclinical Assessment of a New Hybrid Compound C11 Efficacy on Neurogenesis and Cognitive Functions after Pilocarpine Induced Status Epilepticus in Mice
- Source :
- International Journal of Molecular Sciences, Volume 22, Issue 6, International Journal of Molecular Sciences, Vol 22, Iss 3240, p 3240 (2021)
- Publication Year :
- 2021
-
Abstract
- Status epilepticus (SE) is a frequent medical emergency that can lead to a variety of neurological disorders, including cognitive impairment and abnormal neurogenesis. The aim of the presented study was the in vitro evaluation of potential neuroprotective properties of a new pyrrolidine-2,5-dione derivatives compound C11, as well as the in vivo assessment of the impact on the neurogenesis and cognitive functions of C11 and levetiracetam (LEV) after pilocarpine (PILO)-induced SE in mice. The in vitro results indicated a protective effect of C11 (500, 1000, and 2500 ng/mL) on astrocytes under trophic stress conditions in the MTT (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) test. The results obtained from the in vivo studies, where mice 72 h after PILO SE were treated with C11 (20 mg/kg) and LEV (10 mg/kg), indicated markedly beneficial effects of C11 on the improvement of the neurogenesis compared to the PILO control and PILO LEV mice. Moreover, this beneficial effect was reflected in the Morris Water Maze test evaluating the cognitive functions in mice. The in vitro confirmed protective effect of C11 on astrocytes, as well as the in vivo demonstrated beneficial impact on neurogenesis and cognitive functions, strongly indicate the need for further advanced molecular research on this compound to determine the exact neuroprotective mechanism of action of C11.
- Subjects :
- Male
medicine.medical_treatment
Drug Evaluation, Preclinical
Morris water navigation task
Status epilepticus
Pharmacology
Hippocampus
Neuroprotection
Article
Catalysis
Inorganic Chemistry
lcsh:Chemistry
Mice
Cognition
Status Epilepticus
In vivo
medicine
Animals
antiepileptic drugs
Physical and Theoretical Chemistry
Molecular Biology
lcsh:QH301-705.5
Spectroscopy
business.industry
Organic Chemistry
Neurogenesis
General Medicine
Computer Science Applications
pilocarpine
Disease Models, Animal
neurogenesis
Neuroprotective Agents
Anticonvulsant
lcsh:Biology (General)
lcsh:QD1-999
Pilocarpine
Astrocytes
Anticonvulsants
neuroprotection
Levetiracetam
medicine.symptom
business
anticonvulsant
Biomarkers
medicine.drug
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences, Volume 22, Issue 6, International Journal of Molecular Sciences, Vol 22, Iss 3240, p 3240 (2021)
- Accession number :
- edsair.doi.dedup.....20863964371ece488178e345b4648da8