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Synergistic effect of laminin and mesenchymal stem cells on tracheal mucosal regeneration
- Source :
- Biomaterials. 44:134-142
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Although several studies have been successfully undertaken of tracheal reconstruction in terms of the maintaining the framework of the graft, most cases of reconstruction failure have resulted from delayed mucosal regeneration. The purposes of this study were to evaluate whether laminin-coated asymmetrically porous membrane (APM) scaffold enhances mucosal regeneration, to compare the mucosalization capability with mesenchymal stem cell (MSC) seeded APM, and to determine whether laminin coating and MSC seeding has a synergistic effect on mucosal regeneration. We reconstructed the full-thickness anterior tracheal defect of 36 New Zealand White rabbits with the APM scaffold. MSCs were isolated from the rabbit's inguinal fat. The animals were divided into 4 groups by the presence of laminin coating on APM and application of MSC [Group I, -/- (laminin/MSC); Group II, -/+; Group III, +/-; Group IV, +/+]. Endoscopy and histologic evaluation were performed and the results were compared among the groups. The results showed that ciliated columnar epithelium was regenerated earlier in groups II and III than in group I. Furthermore, the application of laminin and MSC had synergistic effects on tracheal epithelial regeneration. These results demonstrate that tracheal reconstruction by laminin-coated APM seeded with MSCs is most effective in enhancing tracheal mucosalization, and appears to be promising strategy in the regenerative treatment of tracheal defects.
- Subjects :
- Pathology
medicine.medical_specialty
Scaffold
Materials science
Group ii
Biophysics
Fluorescent Antibody Technique
Bioengineering
Mesenchymal Stem Cell Transplantation
Biomaterials
Laminin
Porous membrane
medicine
Animals
Humans
Regeneration
Cilia
New zealand white
Mucous Membrane
Staining and Labeling
biology
Regeneration (biology)
Mesenchymal stem cell
Ciliated columnar epithelium
Endoscopy
Mesenchymal Stem Cells
Trachea
Cell Tracking
Mechanics of Materials
Immunology
Ceramics and Composites
biology.protein
Rabbits
Subjects
Details
- ISSN :
- 01429612
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Biomaterials
- Accession number :
- edsair.doi.dedup.....2085e27d05a3cb3ca1911918ab9ac5ac