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Effect of chlorhexidine on the release of lysosomal enzymes from cultured macrophages
- Source :
- Acta Odontologica Scandinavica. 39:285-289
- Publication Year :
- 1981
- Publisher :
- Informa UK Limited, 1981.
-
Abstract
- Exposure of rat's peritoneal macrophages to chlorhexidine at concentrations up to 0.01 per cent resulted in the release of lysosomal enzymes, e.g. chloride-dependent arginine aminopeptidase and beta-D-glucuronidase into the medium from resting cells. The activity of extracellular lactate dehydrogenase increased up to the concentration of 0.01% and then decreased with increasing chlorhexidine concentration. This could indicate a certain type of "mummifying" of the cells and/or their membranes. The incorporation of trypan blue was found in all cells after a rinse with 0.05 per cent or higher concentrations of chlorhexidine. The direct effect of chlorhexidine on these enzyme activities showed a slight activation of beta-D-glucuronidase and lactate dehydrogenase, but an inhibition of arginine aminopeptidase. Also the binding of chlorhexidine to cells was found. Low chlorhexidine concentrations caused an additional enzyme release from phagocytizing cells.
- Subjects :
- Cell Survival
Enzyme release
Biology
Aminopeptidases
chemistry.chemical_compound
Aminopeptidase B
Phagocytosis
Lactate dehydrogenase
medicine
Extracellular
Animals
General Dentistry
Cells, Cultured
Glucuronidase
chemistry.chemical_classification
L-Lactate Dehydrogenase
Macrophages
Chlorhexidine
Rats, Inbred Strains
General Medicine
Rats
Membrane
Enzyme
Biochemistry
chemistry
Female
Trypan blue
Lysosomes
medicine.drug
Subjects
Details
- ISSN :
- 15023850 and 00016357
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Acta Odontologica Scandinavica
- Accession number :
- edsair.doi.dedup.....2082b44fbdc2ffd8de5d663448ddaef6