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Ethanol exposure represses osteogenesis in the developing chick embryo
- Source :
- Reproductive toxicology (Elmsford, N.Y.). 62
- Publication Year :
- 2015
-
Abstract
- It is known that excess alcohol consumption during pregnancy can increase the risk of fetal alcohol spectrum disorder (FASD). However, the effect of ethanol exposure on bone morphogenesis in fetus is largely unknown. In this study, we demonstrated that ethanol treatment of gastrulating chick embryos could inhibit long bone (humerus, radius and ulna) development. Histological examination revealed that ethanol exposure reduced the width of the proliferation and hypertrophic zones. In addition, cell proliferation and alkaline phosphatase activities were repressed. We also investigated the effect on chondrogenesis and chondrogenesis was inhibited. Ethanol exposure also induced excess reactive oxygen species (ROS) production and altered the expression of osteogenesis-related genes. The inhibiting effect on flat bone (sclerotic ossicle) and the generation of cranial neural crest cells (progenitors of craniofacial bones) was also presented. In conclusion, ethanol exposure during the embryonic period retards bone development through excess ROS production and altered bone-associated gene expression.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Long bone
Embryonic Development
Chick Embryo
Biology
Toxicology
Collagen Type XI
Bone and Bones
03 medical and health sciences
0302 clinical medicine
Cranial neural crest
Osteogenesis
Internal medicine
Matrix Metalloproteinase 13
medicine
Animals
Bone morphogenesis
Cell Proliferation
Fetus
Ethanol
Embryogenesis
Embryo
Chondrogenesis
Alkaline Phosphatase
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Matrix Metalloproteinase 9
Neural Crest
Alkaline phosphatase
Reactive Oxygen Species
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 18731708
- Volume :
- 62
- Database :
- OpenAIRE
- Journal :
- Reproductive toxicology (Elmsford, N.Y.)
- Accession number :
- edsair.doi.dedup.....207bf3c93b88776428258c00e9ff0fa1