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Life course programming of stress responses in adolescents and young adults in India: Protocol of the Stress Responses in Adolescence and Vulnerability to Adult Non-communicable disease (SRAVANA) Study
- Source :
- Wellcome Open Research
- Publication Year :
- 2018
-
Abstract
- Background:Early life nutrition may affect individuals’ susceptibility to adult non-communicable diseases (NCD). Psychological stress is a well-recognised NCD risk factor. Recent evidence suggests that impaired foetal nutrition alters neuro-endocrine pathways, and hypothalamic-pituitary-adrenal axis feedback systems, resulting in abnormal stress responses, and NCD risk. This study aims to examine adolescent cortisol and cardiovascular stress responses in relation to maternal nutrition and contemporaneous NCD risk markers.Methods: The study sample will be drawn from three well-established birth cohorts in India; the Parthenon cohort, Mysore (N=550, age~20y), the SARAS KIDS prenatal intervention cohort, Mumbai (N=300, age~10-12y) and the Pune Rural Intervention in Young Adults/ PRIYA cohort, Pune (N=100, age~22y). We will perform the ‘Trier Social Stress Test (TSST)’, a well-accepted stress-test module which involves participants performing 5-minutes each of public speaking and mental arithmetic tasks in front of unfamiliar ‘judges’ (stressor). Repeated measures of salivary cortisol and autonomic cardiovascular outcomes relative to the stressor will be assessed. Measures of psychological stress, cognitive function, blood pressure, glucose-insulin metabolism and depression will be carried out. Mechanistic studies including DNA methylation in gluco-corticoid receptor (NR3C1) and11β-HSD2gene loci and neuroimaging will be carried out in a subsample. Qualitative interviews and focus group discussions in a subsample of the Parthenon cohort will explore the perception of stress and stressors among the youth.We will convert repeated measures into time-weighted averages before analysis. We will carry out multivariable regression analysis to test the associations. We will further refine the analyses using the mixed-model regression and conditional analyses for the association with repeated measures. Ethics and dissemination:This study has been approved by the research ethics committee of CSI Holdsworth Memorial Hospital, Mysore. The findings will be disseminated locally and at international meetings, and reports will be submitted to open access peer reviewed journals.
- Subjects :
- Medicine (miscellaneous)
India
030209 endocrinology & metabolism
General Biochemistry, Genetics and Molecular Biology
TSST
03 medical and health sciences
Study Protocol
0302 clinical medicine
Trier social stress test
Medicine
030212 general & internal medicine
adolescents
Young adult
Risk factor
non-communicable disease
business.industry
Stressor
Repeated measures design
Articles
stress response
Non-communicable disease
medicine.disease
3. Good health
Cohort
Life course approach
business
Clinical psychology
maternal nutrition
Subjects
Details
- ISSN :
- 2398502X
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- Wellcome open research
- Accession number :
- edsair.doi.dedup.....205f4f6b23857f8073be4755db0f00e2