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Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing

Authors :
Yan Yu
Weiming Wang
Fengyu Cong
Hang Liu
Jun Yu
Source :
Sensors (Basel, Switzerland), Sensors, Vol 20, Iss 2092, p 2092 (2020), Sensors, Volume 20, Issue 7
Publication Year :
2020

Abstract

A fiber optic array (FOA) can be used as an alternative or a supplement to the lens in a microscope due to its large magnification, high coupling efficiency and extremely low distortion. Based on our previous research, this paper first demonstrated the resolution and field-of-view (FOV) of the microscope based on the FOA. To further validate the FOA microscope&rsquo<br />s imaging capability, yeast activity and concentration were investigated by simple image processing. The results showed that the percentages of live and dead yeast cells correctly identified were 92.1% and 84.8%, except for the clusters, which agreed well with the manual counting methods. Then, the performances of the portable microscopes based on the FOA and lens were compared and the factors that affect the FOA microscope imaging performance were analyzed.

Details

ISSN :
14248220
Volume :
20
Issue :
7
Database :
OpenAIRE
Journal :
Sensors (Basel, Switzerland)
Accession number :
edsair.doi.dedup.....2059839f53f492589375d54a5885e3b0