Back to Search
Start Over
Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing
- Source :
- Sensors (Basel, Switzerland), Sensors, Vol 20, Iss 2092, p 2092 (2020), Sensors, Volume 20, Issue 7
- Publication Year :
- 2020
-
Abstract
- A fiber optic array (FOA) can be used as an alternative or a supplement to the lens in a microscope due to its large magnification, high coupling efficiency and extremely low distortion. Based on our previous research, this paper first demonstrated the resolution and field-of-view (FOV) of the microscope based on the FOA. To further validate the FOA microscope&rsquo<br />s imaging capability, yeast activity and concentration were investigated by simple image processing. The results showed that the percentages of live and dead yeast cells correctly identified were 92.1% and 84.8%, except for the clusters, which agreed well with the manual counting methods. Then, the performances of the portable microscopes based on the FOA and lens were compared and the factors that affect the FOA microscope imaging performance were analyzed.
- Subjects :
- Optical fiber
Materials science
Microscope
lens
030231 tropical medicine
yeast activity
Magnification
Image processing
portable microscope
02 engineering and technology
Saccharomyces cerevisiae
FOA
lcsh:Chemical technology
Biochemistry
Article
Analytical Chemistry
law.invention
03 medical and health sciences
0302 clinical medicine
Optics
Low distortion
law
Image Processing, Computer-Assisted
Fiber Optic Technology
lcsh:TP1-1185
Electrical and Electronic Engineering
yeast concentration
Instrumentation
Microscopy
Microbial Viability
business.industry
Resolution (electron density)
021001 nanoscience & nanotechnology
Atomic and Molecular Physics, and Optics
Yeast
Lens (optics)
0210 nano-technology
business
Algorithms
Subjects
Details
- ISSN :
- 14248220
- Volume :
- 20
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Sensors (Basel, Switzerland)
- Accession number :
- edsair.doi.dedup.....2059839f53f492589375d54a5885e3b0