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Sulfide-quinone oxidoreductase is required for cysteine synthesis and indispensable to mitochondrial health
- Source :
- Redox Biology, Vol 47, Iss, Pp 102169-(2021), Redox Biology
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- Mitochondrial dysfunction is related to common age-related disorders, including neurodegenerative diseases, metabolic syndrome, and carcinogenesis. Therefore, maintaining the functionality and integrity of mitochondria is important for human health. Herein, we found that sulfide:quinone oxidoreductase (Sqr), which oxidizes hydrogen sulfide to reactive sulfur species (RSS), was indispensable to mitochondria health in the eukaryotic model microorganism Schizosaccharomyces pombe. Sqr knock-out led to morphological changes and functional deficiencies of mitochondria and apoptosis in S. pombe. The Sqr knock-out strain displayed the same phenotypes as the cysteine-synthesis-deficient strain, and cysteine addition complemented the effects caused by Sqr knock-out. In S. pombe, Sqr was the main RSS producer in mitochondria, and RSS instead of H2S was used by cysteine synthase to synthesize cysteine. This finding rewrites the cysteine biosynthesis route in S. pombe and may also in other eukaryotes and prokaryotes, and highlights the importance of cysteine and RSS in maintaining mitochondrial health.<br />Highlights • Sqr is an important RSS producer in mitochondria. • RSS is involved in cysteine de novo biosynthesis. It is the de facto substrate of cysteine synthase. • Sqr is required for maintaining the health of mitochondria, might be a new target for inhibiting cell proliferation.
- Subjects :
- Medicine (General)
QH301-705.5
Hydrogen sulfide
Clinical Biochemistry
chemistry.chemical_element
Sulfides
Mitochondrion
Cysteine synthase
Quinone oxidoreductase
Biochemistry
chemistry.chemical_compound
R5-920
Humans
Cysteine
Quinone Reductases
Biology (General)
Cysteine biosynthesis
Reactive sulfur species
biology
Organic Chemistry
biology.organism_classification
Sulfur
Mitochondria
Sqr
chemistry
Apoptosis
Schizosaccharomyces pombe
biology.protein
Mitochondria health
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 22132317
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- Redox Biology
- Accession number :
- edsair.doi.dedup.....204e5b3f3269e1f616aff62061b0e0a1