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Single-Cell Mass Cytometry on Peripheral Blood Identifies Immune Cell Subsets Associated with Primary Biliary Cholangitis
- Source :
- Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020), Scientific Reports
- Publication Year :
- 2020
- Publisher :
- Cold Spring Harbor Laboratory, 2020.
-
Abstract
- The relationship between Primary Biliary Cholangitis (PBC), a chronic cholestatic autoimmune liver disease, and the peripheral immune system remains to be fully understood. Herein, we performed the first mass cytometry (CyTOF)-based, immunophenotyping analysis of the peripheral immune system in PBC at single-cell resolution. CyTOF was performed on peripheral blood mononuclear cells (PBMCs) from PBC patients (n=33) and age-/sex-matched healthy controls (n=33) to obtain immune cell abundance and marker expression profiles. Hiearchical clustering methods were applied to identify immune cell types and subsets significantly associated with PBC. Subsets of gamma-delta T cells (CD3+TCRgd+), CD8+ T cells (CD3+CD8+CD161+PD1+), and memory B cells (CD3-CD19+CD20+CD24+CD27+) were found to have lower abundance in PBC than in control. In contrast, higher abundance of subsets of monocytes and naïve B cells were observed in PBC compared to control. Furthermore, several naïve B cell (CD3-CD19+CD20+CD24-CD27-) subsets were significantly higher in PBC patients with cirrhosis (indicative of late-stage disease) than in those without cirrhosis. Alternatively, subsets of CD8+CD161+ T cells and memory B cells were lower in abundance in cirrhotic relative to non-cirrhotic PBC patients. Future immunophenotyping investigations could lead to better understanding of PBC pathogenesis and progression, and also to the discovery of novel biomarkers and treatment strategies.
- Subjects :
- 0301 basic medicine
Adult
Male
Naive B cell
lcsh:Medicine
Peripheral blood mononuclear cell
Article
03 medical and health sciences
0302 clinical medicine
Immunophenotyping
Immune system
Humans
Medicine
Mass cytometry
lcsh:Science
skin and connective tissue diseases
030304 developmental biology
CD20
0303 health sciences
Multidisciplinary
biology
Liver Cirrhosis, Biliary
business.industry
lcsh:R
Gastroenterology
Middle Aged
Flow Cytometry
digestive system diseases
Computational biology and bioinformatics
3. Good health
030104 developmental biology
Case-Control Studies
030220 oncology & carcinogenesis
Immunology
biology.protein
Female
lcsh:Q
Single-Cell Analysis
business
Cytometry
CD8
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020), Scientific Reports
- Accession number :
- edsair.doi.dedup.....2043b99850fc7a2dafff53c5b74fbdba