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Cellular SPION Uptake and Toxicity in Various Head and Neck Cancer Cell Lines
- Source :
- Nanomaterials, Volume 11, Issue 3, Nanomaterials, Vol 11, Iss 726, p 726 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Superparamagnetic iron oxide nanoparticles (SPIONs) feature distinct magnetic properties that make them useful and effective tools for various diagnostic, therapeutic and theranostic applications. In particular, their use in magnetic drug targeting (MDT) promises to be an effective approach for the treatment of various diseases such as cancer. At the cellular level, SPION uptake, along with SPION-mediated toxicity, represents the most important prerequisite for successful application. Thus, the present study determines SPION uptake, toxicity and biocompatibility in human head and neck tumor cell lines of the tongue, pharynx and salivary gland. Using magnetic susceptibility measurements, microscopy, atomic emission spectroscopy, flow cytometry, and plasma coagulation, we analyzed the magnetic properties, cellular uptake and biocompatibility of two different SPION types in the presence and absence of external magnetic fields. Incubation of cells with lauric acid and human serum albumin-coated nanoparticles (SPIONLA-HSA) resulted in substantial particle uptake with low cytotoxicity. In contrast, uptake of lauric acid-coated nanoparticles (SPIONLA) was substantially increased but accompanied by higher toxicity. The presence of an external magnetic field significantly increased cellular uptake of both particles, although cytotoxicity was not significantly increased in any of the cell lines. SPIONs coated with lauric acid and/or human serum albumin show different patterns of uptake and toxicity in response to an external magnetic field. Consequently, the results indicate the potential use of SPIONs as vehicles for MDT in head and neck cancer.
- Subjects :
- Biocompatibility
General Chemical Engineering
atomic emission spectroscopy
02 engineering and technology
Article
Flow cytometry
lcsh:Chemistry
03 medical and health sciences
chemistry.chemical_compound
medicine
General Materials Science
ddc:610
Cytotoxicity
030304 developmental biology
0303 health sciences
medicine.diagnostic_test
flow cytometry
superparamagnetic iron oxide nanoparticles
021001 nanoscience & nanotechnology
Human serum albumin
Lauric acid
lcsh:QD1-999
chemistry
Targeted drug delivery
Cell culture
Toxicity
Biophysics
cytotoxicity
holotomographic microscopy
0210 nano-technology
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 20794991
- Database :
- OpenAIRE
- Journal :
- Nanomaterials
- Accession number :
- edsair.doi.dedup.....2042ec38364d1349beee608b94b27722
- Full Text :
- https://doi.org/10.3390/nano11030726