Back to Search
Start Over
A MicroRNA expression profile defining the invasive bladder tumor phenotype
- Source :
- Urologic Oncology: Seminars and Original Investigations. 29:794-801.e1
- Publication Year :
- 2011
- Publisher :
- Elsevier BV, 2011.
-
Abstract
- Objective The purpose of this study was to identify microRNA (miRNA) involved in the transition between the noninvasive and invasive urothelial carcinoma of the bladder (UCB) phenotype. Methods Differential expression of miRNA was identified in a microarray format between noninvasive and invasive UCB cell lines and confirmed using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) within this cell panel. Normalization of qRT-PCR with miR-222 was established from the microarray data and validated within a panel of 57 UCB tumors (26 noninvasive lesions (Ta/G1) and 31 invasive lesions (T2-T4). Pre-miR constructs were transfected into appropriate UCB cell lines to establish a change in invasive potential. Results Differential expression of miRNAs was identified from microarray analysis and included reduced expression associated with miR-30b, miR-31, miR-141, miR-200a, miR-200b, miR-200c, miR-205, miR-21 in invasive lesions and elevated miR-99a in noninvasive UCB lesions. Reduced invasion potential was recorded in UM-UC-3, following pre-miR transfection, in all UCB cell lines with the exception of UM-UC-3/miR-30b transfectants. Our results identify a panel of miRNA modulated and expressed in invasive UCB tumors and demonstrates a role for them in the invasive phenotype. Conclusions The diagnostic test, based on the three most discriminatory miRNAs in our panel (miR-200c, miR-141, and miR-30b), showed a sensitivity of 100% and a specificity of 96.2%. Such a panel of miRNAs has the potential to identify invasive bladder tumors misclassified in pathologic assessment of bladder biopsy specimens.
- Subjects :
- Pathology
medicine.medical_specialty
Genotype
Microarray
Invasive urothelial carcinoma
Urology
Kaplan-Meier Estimate
Real-Time Polymerase Chain Reaction
microRNA
medicine
Humans
Neoplasm Invasiveness
Oligonucleotide Array Sequence Analysis
Carcinoma, Transitional Cell
Bladder cancer
Reverse Transcriptase Polymerase Chain Reaction
business.industry
Microarray analysis techniques
Gene Expression Profiling
MicroRNA Expression Profile
medicine.disease
Gene expression profiling
MicroRNAs
Real-time polymerase chain reaction
Urinary Bladder Neoplasms
Oncology
embryonic structures
business
Subjects
Details
- ISSN :
- 10781439
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Urologic Oncology: Seminars and Original Investigations
- Accession number :
- edsair.doi.dedup.....2033fe1d07bb3fc4af8605850234d97e