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Presence of autocrine hepatocyte growth factor-Met signaling and its role in proliferation and migration of SNU-484 gastric cancer cell line
- Source :
- Experimental & Molecular Medicine. 37:213-219
- Publication Year :
- 2005
- Publisher :
- Springer Science and Business Media LLC, 2005.
-
Abstract
- Autocrine stimulation via coexpression of hepatocyte growth factor (HGF) and its receptor (Met) has been reported in many human sarcomas, but few in carcinomas. In this report, we found that one gastric cancer cell line, SNU-484, among 11 gastric cell lines tested has an autocrine HGF- Met stimulation. RT-PCR, ELISA and scattering assay using MDCK cells revealed that SNU-484 cells secreted a significant amount of active HGF (about 1.25 +/- 0.41 ng/24 h/10(6) cells) into conditioned medium. Resultantly, Met in this cell line was constitutively phosphorylated. Neutralizing antibodies against HGF reduced the tyrosine phosphorylation of Met, resulting in the inhibition of cell proliferation and migration (P
- Subjects :
- medicine.medical_specialty
Antibodies, Neoplasm
Clinical Biochemistry
Enzyme-Linked Immunosorbent Assay
Autocrine Communication
Biochemistry
chemistry.chemical_compound
Dogs
Cell Movement
Neutralization Tests
Stomach Neoplasms
Internal medicine
Tumor Cells, Cultured
medicine
Animals
Phosphorylation
Receptor
Autocrine signalling
Molecular Biology
Cell Proliferation
Hepatocyte Growth Factor
Reverse Transcriptase Polymerase Chain Reaction
Chemistry
Cell growth
Tyrosine phosphorylation
Proto-Oncogene Proteins c-met
Endocrinology
Cell culture
Culture Media, Conditioned
Cancer research
Tyrosine
Molecular Medicine
Hepatocyte growth factor
medicine.drug
Subjects
Details
- ISSN :
- 20926413
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Experimental & Molecular Medicine
- Accession number :
- edsair.doi.dedup.....2032a9f9d1538cfe6362da6ba9a32de3