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A simple highly specific fluorescent probe for simultaneous discrimination of cysteine/homocysteine and glutathione/hydrogen sulfide in living cells and zebrafish using two separated fluorescence channels under single wavelength excitation
- Source :
- The Analyst. 144(14)
- Publication Year :
- 2019
-
Abstract
- Biothiols such as cysteine (Cys), homocysteine (Hcy), glutathione (GSH) and hydrogen sulfide (H2S) are widely found in mammalian cells. They are closely related to the production and metabolic pathways and play very important roles in physiological and pathological activities. Therefore, the quantitative detection of these biothiols is of great significance. Although many fluorescent probes have been successfully used to track biothiols in biological samples, the fluorescence method for simultaneously detecting these biothiols using separated fluorescence emission channels under single wavelength excitation is still immature. In this work, we prepared the conjugate of seminaphthorhodafluor (SNARF) dye and 7-nitro-1,2,3-benzoxadiazole (NBD) using as a simple long-wavelength fluorescent probe SNARF-NBD for specific detection of biothiols. Cys/Hcy and GSH/H2S were identified by two separated fluorescence emission channels under single wavelength excitation, which showed good selectivity and sensitivity. In addition, SNARF-NBD has low cytotoxicity and shows good imaging ability in living cells and zebrafish.
- Subjects :
- Hydrogen sulfide
02 engineering and technology
01 natural sciences
Biochemistry
Analytical Chemistry
chemistry.chemical_compound
Mice
Electrochemistry
Environmental Chemistry
Animals
Cysteine
Hydrogen Sulfide
Cytotoxicity
Zebrafish
Homocysteine
Spectroscopy
Fluorescent Dyes
Oxadiazoles
biology
Rhodamines
010401 analytical chemistry
Optical Imaging
Glutathione
021001 nanoscience & nanotechnology
biology.organism_classification
Fluorescence
0104 chemical sciences
RAW 264.7 Cells
Spectrometry, Fluorescence
chemistry
Biophysics
0210 nano-technology
Selectivity
Conjugate
Subjects
Details
- ISSN :
- 13645528
- Volume :
- 144
- Issue :
- 14
- Database :
- OpenAIRE
- Journal :
- The Analyst
- Accession number :
- edsair.doi.dedup.....201d0e9614a0f2ddbfc6aac9e2b95d32