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Effects of vitamin A deficiency in the postnatal mouse heart: role of hepatic retinoid stores
- Source :
- American Journal of Physiology-Heart and Circulatory Physiology. 310:H1773-H1789
- Publication Year :
- 2016
- Publisher :
- American Physiological Society, 2016.
-
Abstract
- To determine whether hepatic depletion of vitamin A (VA) stores has an effect on the postnatal heart, studies were carried out with mice lacking liver retinyl ester stores fed either a VA-sufficient (LRVAS) or VA-deficient (LRVAD) diet (to deplete circulating retinol and extrahepatic stores of retinyl esters). There were no observable differences in the weights or gross morphology of hearts from LRVAS or LRVAD mice relative to sex-matched, age-matched, and genetically matched wild-type (WT) controls fed the VAS diet (WTVAS), but changes in the transcription of functionally relevant genes were consistent with a state of VAD in LRVAS and LRVAD ventricles. In silico analysis revealed that 58/67 differentially expressed transcripts identified in a microarray screen are products of genes that have DNA retinoic acid response elements. Flow cytometric analysis revealed a significant and cell-specific increase in the number of proliferating Sca-1 cardiac progenitor cells in LRVAS animals relative to WTVAS controls. Before myocardial infarction, LRVAS and WTVAS mice had similar cardiac systolic function and structure, as measured by echocardiography, but, unexpectedly, repeat echocardiography demonstrated that LRVAS mice had less adverse remodeling by 1 wk after myocardial infarction. Overall, the results demonstrate that the adult heart is responsive to retinoids, and, most notably, reducing hepatic VA stores (while maintaining circulating levels of VA) impacts ventricular gene expression profiles, progenitor cell numbers, and response to injury.
- Subjects :
- 0301 basic medicine
Vitamin
medicine.medical_specialty
Microarray
Receptors, Retinoic Acid
Physiology
medicine.drug_class
Myocardial Infarction
Retinoic acid
Mice
Retinoids
03 medical and health sciences
chemistry.chemical_compound
Integrative Cardiovascular Physiology and Pathophysiology
Physiology (medical)
Internal medicine
Animals
Medicine
Retinoid
Myocardial infarction
Progenitor cell
Mice, Knockout
Vitamin A Deficiency
business.industry
Myocardium
Retinol
Heart
medicine.disease
Vitamin A deficiency
030104 developmental biology
Endocrinology
Liver
chemistry
Echocardiography
Cardiology and Cardiovascular Medicine
business
Acyltransferases
Subjects
Details
- ISSN :
- 15221539 and 03636135
- Volume :
- 310
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Heart and Circulatory Physiology
- Accession number :
- edsair.doi.dedup.....201c1f023a30603a1e5da65703991204
- Full Text :
- https://doi.org/10.1152/ajpheart.00887.2015