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Arginine-conjugated polypropylenimine dendrimer as a non-toxic and efficient gene delivery carrier
- Source :
- Biomaterials. 28(11)
- Publication Year :
- 2006
-
Abstract
- We synthesized arginine-conjugated polypropylenimine dendrimer G2 (DAB-8), PPI2-R for gene delivery systems. Synthesized PPI2-R could retard plasmid DNA at a weight ratio of 4 completely and PPI2-R polyplexes showed a fluorescence of less than 10% over a charge ratio of 2 by PicoGreen reagent assay, suggesting its good DNA condensing ability. The size of PPI2-R polyplex was measured to about 200nm at a charge ratio of 150. PPI2-R displayed 80-90% cell viability at even a 150microg/mL concentration. Transfection efficiency of PPI2-R was found to be high comparable to that of PEI25kD and to be 8-214 times higher than that of unmodified PPI2 on HeLa and 293 cells. Moreover, PPI2-R showed 4 times higher transfection efficiency than PEI25kD, treating with 10microg pDNA because of its low cytotoxicity on HeLa cells. Finally, PPI2-R showed a transfection efficiency 2-3 times higher than PEI25kD on HUVECs, showing its potency as a gene delivery carrier for primary cells. These results demonstrate that arginine-conjugation of PPI2 is successful in developing a low toxic and highly transfection efficient gene delivery carrier.
- Subjects :
- Dendrimers
Magnetic Resonance Spectroscopy
Cell Survival
Biophysics
Bioengineering
Gene delivery
Biology
Arginine
Transfection
Cell Line
Biomaterials
HeLa
Drug Delivery Systems
Dendrimer
Polyamines
Humans
Viability assay
Organic Chemicals
Cytotoxicity
Drug Carriers
HEK 293 cells
Gene Transfer Techniques
DNA
biology.organism_classification
Molecular biology
Models, Chemical
Mechanics of Materials
Ceramics and Composites
Endothelium, Vascular
Drug carrier
HeLa Cells
Subjects
Details
- ISSN :
- 01429612
- Volume :
- 28
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Biomaterials
- Accession number :
- edsair.doi.dedup.....1fef3512a201f73f33c3ae763d1a4468