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HHV-5 epitope: A potential vaccine candidate with high antigenicity and large coverage
- Source :
- Journal of Biomolecular Structure and Dynamics. 37:2098-2109
- Publication Year :
- 2018
- Publisher :
- Informa UK Limited, 2018.
-
Abstract
- Outbreak of Human Herpes virus-5 (HHV-5) infection in emerging countries has raised worldwide health concern owing to prevalence of congenital impairments and life threatening consequences in immunocompromised individuals. Thus, there lies an impending need to develop vaccine against HHV-5. HHV-5 enters into host cells with the help of necessary components glycoprotein B (gB) and H/L. In this study, the conformational linear B-cell and T-cell epitopes for gB of HHV-5 have been predicted using conformational approaches, for their possible collective use as vaccine candidates. We examined epitope's interactions with major histocompatibility complexes using molecular docking and also investigated their stable binding with specific toll like receptor-2 (TLR2), present on host cells during HHV-5 infection. Predicted MHC-I epitope 'LVAIAVVII' with high antigenicity and large coverage of HLA alleles was found to superimpose on MHC-II epitope (Rank 1) and was also identified to be the core sequence of putative B cell epitope 'ILVAIAVVIITYLI'. Resulting epitope was found to have consistent interaction with TLR2 during long term (100 ns) MD run. We also validated this nonamer epitope for its dissimilarity with human genome and high population coverage, suggesting it to be a potential vaccine candidate with higher coverage for both the MHC alleles of Indian population. Communicated by Ramaswamy H. Sarma.
- Subjects :
- Models, Molecular
0301 basic medicine
Antigenicity
viruses
Cytomegalovirus
Epitopes, T-Lymphocyte
India
Herpesvirus Vaccines
Molecular Dynamics Simulation
Biology
Epitope
03 medical and health sciences
0302 clinical medicine
Viral Envelope Proteins
Structural Biology
Human herpes
Humans
Glycoprotein B
Antigens, Viral
Molecular Biology
Genome, Human
Human herpes virus
Outbreak
T-Lymphocytes, Helper-Inducer
General Medicine
biochemical phenomena, metabolism, and nutrition
Virology
Toll-Like Receptor 2
Molecular Docking Simulation
030104 developmental biology
030220 oncology & carcinogenesis
Epitopes, B-Lymphocyte
T-Lymphocytes, Cytotoxic
Subjects
Details
- ISSN :
- 15380254 and 07391102
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Journal of Biomolecular Structure and Dynamics
- Accession number :
- edsair.doi.dedup.....1fe9629f8069bb9577726840b3d9591b