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Metabolomics characterization of energy metabolism reveals glycogen accumulation in gut-microbiota-lacking mice
- Source :
- The Journal of nutritional biochemistry. 23(7)
- Publication Year :
- 2010
-
Abstract
- Microbiota in the gut are considered an important environmental factor associated with host metabolism and physiology. Although gut microbiota are known to contribute to hepatic lipogenesis and fat storage, little is known about how the condition influences the deposition of glycogen in the liver. To better understand and characterize the host energy metabolism in guts lacking microbiota, we compared the liver metabolome of specific pathogen-free and germ-free mice by gas chromatography-mass spectrometry combined with partial least-squares discriminant analysis. We identified 30 of 52 highly reproducible peaks in chromatograms of liver tissue extracts from the two groups of mice. The two groups showed significant differences in metabolic profile. Changes in liver metabolism involved metabolites such as amino acids, fatty acids, organic acids and carbohydrates. The metabolic profile of germ-free mice suggests that they synthesize glycogen and accumulate it in the liver through gluconeogenesis and glycogenesis. Our findings shed light on a new perspective of the role of gut microbiota in energy metabolism and will be useful to help study probiotics, obesity and metabolic diseases.
- Subjects :
- Male
Endocrinology, Diabetes and Metabolism
Clinical Biochemistry
Carbohydrates
Biology
Gut flora
Biochemistry
Gas Chromatography-Mass Spectrometry
chemistry.chemical_compound
Mice
Metabolomics
Metabolome
medicine
Glycogen storage disease
Animals
Germ-Free Life
Amino Acids
Molecular Biology
Nutrition and Dietetics
Glycogen
Fatty Acids
Gluconeogenesis
Discriminant Analysis
Metabolism
biology.organism_classification
medicine.disease
Gastrointestinal Tract
Mice, Inbred C57BL
chemistry
Liver
Glycogenesis
Metagenome
Energy Metabolism
Subjects
Details
- ISSN :
- 18734847
- Volume :
- 23
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- The Journal of nutritional biochemistry
- Accession number :
- edsair.doi.dedup.....1fe5c40286d245fc9fc0e2f92db9fd61