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Indirect immunofluorescence test and enzyme-linked immunosorbent assay for detection of Campylobacter pylori
Indirect immunofluorescence test and enzyme-linked immunosorbent assay for detection of Campylobacter pylori
- Source :
- Journal of clinical microbiology. 27(2)
- Publication Year :
- 1989
-
Abstract
- An indirect immunofluorescence test (IIF) has been developed for detecting Campylobacter pylori in gastroduodenal biopsies. This test was compared with standard methods of C. pylori diagnosis, namely Gram staining and urease test, in a study population of 226 patients; 121 of the biopsy specimens were cultured for C. pylori as well. C. pylori colonization was detected in 154 of 226 patients (68%) by at least one of these methods (IIF, 96%; Gram staining, 78%; urease test, 60%; cultivation, 55%). Serum samples from 191 patients of the study population were screened for circulating antibodies to C. pylori by an indirect enzyme-linked immunosorbent assay with whole, untreated bacteria as antigen. Of these serum specimens, 140 (73%) revealed absorbance readings above the limit of positivity, which was determined as an optical density of greater than 0.35 at 405/620 nm. Of 132 serum specimens, 128 (97%) from patients with C. pylori detected in biopsies, but only 12 (20%) of 59 specimens from those without C. pylori detection showed elevated specific antibody levels. Our data revealed that IIF proved to be the superior rapid, sensitive, and specific diagnostic method. The correlation between microbiological findings and the immune response favors our enzyme-linked immunosorbent assay as an additional tool in C. pylori diagnosis.
- Subjects :
- Microbiology (medical)
Adult
Male
Adolescent
Duodenum
Spirillaceae
Biopsy
Rapid urease test
Fluorescent Antibody Technique
Enzyme-Linked Immunosorbent Assay
medicine.disease_cause
law.invention
Microbiology
Antigen
law
medicine
Humans
Child
Aged
Aged, 80 and over
biology
medicine.diagnostic_test
Campylobacter
Stomach
IIf
Endoscopy
Middle Aged
biology.organism_classification
Antibodies, Bacterial
Gram staining
biology.protein
Female
Antibody
Research Article
Subjects
Details
- ISSN :
- 00951137
- Volume :
- 27
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of clinical microbiology
- Accession number :
- edsair.doi.dedup.....1fd09798a8ea2face9d4fc02901fc595