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Transcriptome Analysis Reveals Different Responsive Patterns to Nitrogen Deficiency in Two Wheat Near-Isogenic Lines Contrasting for Nitrogen Use Efficiency
- Source :
- Biology, Vol 10, Iss 1126, p 1126 (2021), Biology, Volume 10, Issue 11
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Simple Summary Nitrogen (N) limitation is the key factor for wheat production worldwide. Therefore, the development of genotypes with improved nitrogen use efficiency (NUE) is a prerequisite for sustainable and productive agriculture. Exploring the molecular mechanisms of low N stress tolerance is significant for breeding wheat cultivars with high NUE. To clarify the underlying molecular mechanisms of enhanced resilience to low N in high-NUE wheat, we performed an RNA sequencing (RNA-seq) analysis. In the current research, two wheat near-isogenic lines (NILs) differing dramatically in NUE were used to measure gene expression differences under different N treatments. There was a dramatic difference between two wheat NILs in response to N deficiency at the transcriptional level, and the classification of identified candidate genes may provide new valuable insights into the resilience mechanism of wheat. Abstract The development of crop cultivars with high nitrogen use efficiency (NUE) under low-N fertilizer inputs is imperative for sustainable agriculture. However, there has been little research on the molecular mechanisms underlying enhanced resilience to low N in high-NUE plants. The comparison of the transcriptional responses of genotypes contrasting for NUE will facilitate an understanding of the key molecular mechanism of wheat resilience to low-N stress. In the current study, the RNA sequencing (RNA-seq) technique was employed to investigate the genotypic difference in response to N deficiency between two wheat NILs (1Y, high-NUE, and 1W, low-NUE). In our research, high- and low-NUE wheat NILs showed different patterns of gene expression under N-deficient conditions, and these N-responsive genes were classified into two major classes, including “frontloaded genes” and “relatively upregulated genes”. In total, 103 and 45 genes were identified as frontloaded genes in high-NUE and low-NUE wheat, respectively. In summary, our study might provide potential directions for further understanding the molecular mechanism of high-NUE genotypes adapting to low-N stress.
- Subjects :
- Genetics
General Immunology and Microbiology
QH301-705.5
Nitrogen deficiency
food and beverages
RNA
qRT-PCR
RNA sequencing (RNA-seq)
Biology
Article
General Biochemistry, Genetics and Molecular Biology
Transcriptome
Crop
nitrogen deficiency
Gene expression
Genotype
nitrogen use efficiency (NUE)
near-isogenic lines (NILs)
Cultivar
Biology (General)
General Agricultural and Biological Sciences
Gene
Subjects
Details
- ISSN :
- 20797737
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Biology
- Accession number :
- edsair.doi.dedup.....1fcf545c7c7072a27dbde5ec89056fd0