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Propagation of the Microsporidian Parasite Edhazardia aedis in Aedes aegypti Mosquitoes

Authors :
Neil D. Sanscrainte
Brendan J Kelly
James J. Becnel
Sarah M. Short
Anthony Grigsby
Source :
Journal of Visualized Experiments.
Publication Year :
2020
Publisher :
MyJove Corporation, 2020.

Abstract

Edhazardia aedis is a microsporidian parasite of Aedes aegypti mosquitoes, a disease vector that transmits multiple arboviruses which cause millions of disease cases each year. E. aedis causes mortality and reduced reproductive fitness in the mosquito vector and has been explored for its potential as a biocontrol agent. The protocol we present for culturing E. aedis is based on its natural infection cycle, which involves both horizontal and vertical transmission at different life stages of the mosquito host. Ae. aegypti mosquitoes are exposed to spores in the larval stage. These infected larvae then mature into adults and transmit the parasite vertically to their offspring. Infected offspring are then used as a source of spores for future horizontal transmission. Culturing E. aedis can be challenging to the uninitiated given the complexities of the parasite's life cycle, and this protocol provides detailed guidance and visual aids for clarification.

Details

ISSN :
1940087X
Database :
OpenAIRE
Journal :
Journal of Visualized Experiments
Accession number :
edsair.doi.dedup.....1fc549386104b75f8848b5229e57dfb5