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Investigating nanostructured liquid crystalline particles as prospective ocular delivery vehicle for tobramycin sulfate: Ex vivo and in vivo studies
- Source :
- Journal of Advanced Pharmaceutical Technology & Research, Vol 12, Iss 4, Pp 356-361 (2021), Journal of Advanced Pharmaceutical Technology & Research
- Publication Year :
- 2021
- Publisher :
- Wolters Kluwer Medknow Publications, 2021.
-
Abstract
- Tobramycin remains the anchor drug for bacterial keratitis treatment and management; however, unlike other aminoglycosides, it does not pass through the gastrointestinal tract. The aim of the current investigation was to formulate tobramycin-loaded nanostructured liquid crystalline particles as an ophthalmic drug delivery system to ameliorate its preocular residence duration and ophthalmic bioavailability. Tobramycin cubosomes were fabricated by liquid–lipid monoolein, water, and poloxamer 407 as a stabilizer. Corneal penetration studies exhibited that the apparent permeation coefficient of tobramycin cubosomes was nearly 3.6-fold greater than marketed tobramycin eye drops. Ocular in vivo analysis performed in rabbits' eyes manifested that the intensity of bacterial keratitis was reduced on day 3, and on day 5, the manifestations were considerably mitigated with tobramycin cubosomes as compared to marked eye drops. Pharmacokinetic study of rabbit aqueous humor demonstrated that the area under curve and the peak concentration of optimized cubosomes were 3.1-fold and 3.3-fold, respectively, which was significantly higher than marketed eye drops. Moreover, histopathological studies illustrated the existence of normal ocular structures, thus indicating that there was no damage to the corneal epithelium or stromal layer. Consequently, the results acquired demonstrated that tobramycin-loaded cubosomal formulation could be a propitious lipid-based nanodelivery system that would enhance retention time and corneal permeability contrast to commercial eye drops.
- Subjects :
- genetic structures
RM1-950
hen's egg chorioallantoic membrane test
Pharmacy and materia medica
Pharmacokinetics
In vivo
medicine
Tobramycin
nanostructured liquid crystalline particles
cubosomes
Corneal epithelium
Chemistry
bacterial keratitis
Tobramycin Sulfate
eye diseases
Bioavailability
RS1-441
medicine.anatomical_structure
ocular pharmacokinetic studies
Poloxamer 407
Original Article
sense organs
Therapeutics. Pharmacology
Ex vivo
Biomedical engineering
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 09762094 and 22314040
- Volume :
- 12
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of Advanced Pharmaceutical Technology & Research
- Accession number :
- edsair.doi.dedup.....1fc067757a480dca247f114162a2dc6c