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Development of a recombinant vaccine containing a spike S1-Fc fusion protein induced protection against MERS-CoV in human DPP4 knockin transgenic mice
- Source :
- Journal of Virological Methods
- Publication Year :
- 2021
- Publisher :
- Published by Elsevier B.V., 2021.
-
Abstract
- The Middle East respiratory syndrome coronavirus (MERS-CoV), belonging to the family Coronaviridae and genus Betacoronavirus, has been recognized as a highly pathogenic virus. Due to the lack of therapeutic or preventive agents against MERS-CoV, developing an effective vaccine is essential for preventing a viral outbreak. To address this, we developed a recombinant S1 subunit of MERS-CoV spike protein fused with the human IgG4 Fc fragment (LV-MS1-Fc) in Chinese hamster ovary (CHO) cells. Thereafter, we identified the baculovirus gp64 signal peptide-directed secretion of LV-MS1-Fc protein in the extracellular fluid. To demonstrate the immunogenicity of the recombinant LV-MS1-Fc proteins, BALB/c mice were inoculated with 2.5 μg of LV-MS1-Fc. The inoculated mice demonstrated a significant humoral immune response, measured via total IgG and neutralizing antibodies. In addition, human dipeptidyl peptidase-4 (DPP4) transgenic mice vaccinated with LV-MS1-Fc showed the protective capacity of LV-MS1-Fc against MERS-CoV with no inflammatory cell infiltration. These data showed that the S1 and Fc fusion protein induced potent humoral immunity and antigen-specific neutralizing antibodies in mice, and conferred protection against coronavirus viral challenge, indicating that LV-MS1-Fc is an effective vaccine candidate against MERS-CoV infection.
- Subjects :
- viruses
medicine.disease_cause
Antibodies, Viral
Mice
PCR, polymerase chain reaction
Cricetinae
Alum adjuvant
Fc, fragment of crystalline
Coronavirus
Fragment of crystalline (Fc) molecule
Mice, Inbred BALB C
Vaccines, Synthetic
biology
Immunogenicity
Chinese hamster ovary cell
RBD, receptor-binding domain
MERS-CoV, Middle East respiratory syndrome coronavirus
RLU, relative light unit
ELISA, enzyme-linked immunosorbent assay
Spike Glycoprotein, Coronavirus
Middle East Respiratory Syndrome Coronavirus
Antibody
Coronavirus Infections
BSL, biosafety level
Dipeptidyl Peptidase 4
SARS-CoV, severe acute respiratory syndrome coronavirus
PBS, phosphate-buffered saline
Mice, Transgenic
CHO Cells
CHO, Chinese hamster ovary
Virus
Article
Vaccine development
Immune system
Cricetulus
Virology
medicine
SFM, serum-free medium
Animals
Humans
CEF, chicken embryo fibroblast
MERS, Middle East respiratory syndrome
PBST, phosphate-buffered saline in 0.05 % Tween 20
Viral Vaccines
Antibodies, Neutralizing
Humoral immunity
biology.protein
PFU, plaque-forming unit
Subjects
Details
- Language :
- English
- ISSN :
- 18790984 and 01660934
- Database :
- OpenAIRE
- Journal :
- Journal of Virological Methods
- Accession number :
- edsair.doi.dedup.....1fa135d80e2979326810f3356d538ed5