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Application of the FICTION technique for the simultaneous detection of immunophenotype and chromosomal abnormalities in routinely fixed, paraffin wax embedded bone marrow trephines
- Publication Year :
- 2016
-
Abstract
- The use of interphase fluorescence in situ hybridisation (FISH) to study cytogenetic abnormalities in routinely fixed paraffin wax embedded tissue has become commonplace over the past decade. However, very few studies have applied FISH to routinely fixed bone marrow trephines (BMTs). This may be because of the acid based decalcification methods that are commonly used during the processing of BMTs, which may adversely affect the suitability of the sample for FISH analysis. For the first time, this report describes the simultaneous application of FISH and immunofluorescent staining (the FICTION technique) to formalin fixed, EDTA decalcified and paraffin wax embedded BMTs. This technique allows the direct correlation of genetic abnormalities to immunophenotype, and therefore will be particularly useful for the identification of genetic abnormalities in specific tumour cells present in BMTs. The application of this to routine clinical practice will assist diagnosis and the detection of minimal residual disease.
- Subjects :
- Pathology
medicine.medical_specialty
cytogenetic abnormalities
FISH
FICTION
bone marrow trephines
immunofluorescent staining
Biopsy
Biology
Immunophenotyping
Pathology and Forensic Medicine
Short Reports
Antigens, Neoplasm
Paraffin wax
Formaldehyde
medicine
Humans
Edetic Acid
In Situ Hybridization, Fluorescence
Chromosome Aberrations
Paraffin Embedding
medicine.diagnostic_test
Bone decalcification
Bone Marrow Examination
General Medicine
Antigens, CD20
Minimal residual disease
Staining
Bone marrow examination
medicine.anatomical_structure
Hematologic Neoplasms
Bone marrow
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....1f9968a84b31fcf44e72a5ef70fb3039