Back to Search
Start Over
Enhanced Diagnostic Yields of Bacteremia and Candidemia in Blood Specimens by PCR-Electrospray Ionization Mass Spectrometry
- Source :
- Journal of Clinical Microbiology. 51:3535-3541
- Publication Year :
- 2013
- Publisher :
- American Society for Microbiology, 2013.
-
Abstract
- A prospective study was performed to determine the value of direct molecular testing of whole blood for detecting the presence of culturable and unculturable bacteria and yeasts in patients with suspected bloodstream infections. A total of 464 adult and pediatric patients with positive blood cultures matched with 442 patients with negative blood cultures collected during the same period were recruited during a 10-month study. PCR amplification coupled with electrospray ionization mass spectrometry (PCR-ESI-MS) plus blood culture reached an overall agreement of 78.6% in the detection and species-level identification of bacterial and candidal pathogens. Of 33 culture-negative/PCR-ESI-MS-positive specimens, 31 (93.9%) were judged to be truly bacteremic and/or candidemic based on a medical chart review and analytical metrics. Among the 15 culture-positive specimens in which PCR-ESI-MS detected additional bacterial or yeast species, 66.7% and 20.0% of the additional positive specimens by PCR-ESI-MS were judged to be truly or possibly bacteremic and/or candidemic, respectively. Direct analysis of blood samples by PCR-ESI-MS rapidly detects bacterial and yeast pathogens in patients with bloodstream infections. When used in conjunction with blood culture, PCR-ESI-MS enhances the diagnostics of septicemia by shortening test turnaround time and improving yields.
- Subjects :
- Adult
Male
Microbiological Techniques
Microbiology (medical)
Spectrometry, Mass, Electrospray Ionization
Time Factors
Electrospray ionization
Bacteremia
Biology
Mass spectrometry
Polymerase Chain Reaction
law.invention
Microbiology
law
medicine
Humans
Blood culture
Prospective Studies
Prospective cohort study
Polymerase chain reaction
Aged
Candida
Whole blood
Bacteria
medicine.diagnostic_test
Candidemia
Bacteriology
Middle Aged
biology.organism_classification
medicine.disease
Blood
Molecular Diagnostic Techniques
Female
Subjects
Details
- ISSN :
- 1098660X and 00951137
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Microbiology
- Accession number :
- edsair.doi.dedup.....1f7efcc4e2d51a6d1804a083cd85bd91