Back to Search Start Over

Identification and analysis of U5 snRNA variants in Drosophila

Authors :
Donald C. Rio
Enbo Ma
Susan E. Celniker
Dennis J. Lullo
Jennifer A. Doudna
Li Chen
Source :
RNA. 11:1473-1477
Publication Year :
2005
Publisher :
Cold Spring Harbor Laboratory, 2005.

Abstract

Distinct isoforms of spliceosomal RNAs may be involved in regulating pre-messenger RNA splicing in eukaryotic cells. During a large-scale effort to identify small noncoding RNAs in Drosophila, we isolated a U5 snRNA-like molecule containing a 5′ segment identical to that of the canonical (major) U5 snRNA but with a variant Sm binding site and a distinct 3′ hairpin sequence. Based on this finding, another six similar U5 snRNA-like sequences were identified within the Drosophila genome by sequence similarity to the invariant loop in the 5′ half of U5. Interestingly, although all of these variants are expressed in vivo, each shows a distinct temporal expression profile during Drosophila development, and one is expressed primarily in fly heads. The presence of these U5 snRNA variants within RNP particles suggests their role in splicing and implies a possible connection to regulation of developmental and tissue-specific gene expression.

Details

ISSN :
14699001 and 13558382
Volume :
11
Database :
OpenAIRE
Journal :
RNA
Accession number :
edsair.doi.dedup.....1f704333f12a83e707a765895094ba3e
Full Text :
https://doi.org/10.1261/rna.2141505