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Identification and analysis of U5 snRNA variants in Drosophila
- Source :
- RNA. 11:1473-1477
- Publication Year :
- 2005
- Publisher :
- Cold Spring Harbor Laboratory, 2005.
-
Abstract
- Distinct isoforms of spliceosomal RNAs may be involved in regulating pre-messenger RNA splicing in eukaryotic cells. During a large-scale effort to identify small noncoding RNAs in Drosophila, we isolated a U5 snRNA-like molecule containing a 5′ segment identical to that of the canonical (major) U5 snRNA but with a variant Sm binding site and a distinct 3′ hairpin sequence. Based on this finding, another six similar U5 snRNA-like sequences were identified within the Drosophila genome by sequence similarity to the invariant loop in the 5′ half of U5. Interestingly, although all of these variants are expressed in vivo, each shows a distinct temporal expression profile during Drosophila development, and one is expressed primarily in fly heads. The presence of these U5 snRNA variants within RNP particles suggests their role in splicing and implies a possible connection to regulation of developmental and tissue-specific gene expression.
- Subjects :
- Spliceosome
RNA Splicing
Molecular Sequence Data
Prp24
Biology
Autoantigens
Protein Structure, Secondary
snRNP Core Proteins
RNA, Small Nuclear
Animals
Protein Isoforms
snRNP
Amino Acid Sequence
Cloning, Molecular
Letter to the Editor
Molecular Biology
Ribonucleoprotein
Genetics
Binding Sites
Base Sequence
SnRNP Core Proteins
Gene Expression Profiling
Genetic Variation
RNA
Ribonucleoproteins, Small Nuclear
RNA splicing
Spliceosomes
Nucleic Acid Conformation
Drosophila
Small nuclear RNA
Subjects
Details
- ISSN :
- 14699001 and 13558382
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- RNA
- Accession number :
- edsair.doi.dedup.....1f704333f12a83e707a765895094ba3e
- Full Text :
- https://doi.org/10.1261/rna.2141505