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Rapid immunosurveillance by recirculating lymphocytes in the rat intestine: critical role of unsulfated sialyl-Lewis X on high endothelial venules of the Peyer’s patches
- Source :
- International Immunology
- Publication Year :
- 2018
- Publisher :
- Oxford University Press (OUP), 2018.
-
Abstract
- Lymphocytes can transfer from blood to lymph or respond to antigen in Peyer’s patches<br />Naive lymphocytes systemically recirculate for immunosurveillance inspecting foreign antigens and pathogens in the body. Trafficking behavior such as the migration pathway and transit time within the gastrointestinal tract, however, remains to be elucidated. Rat thoracic duct lymphocytes (TDLs) were transferred to a congeneic host that had undergone mesenteric lymphadenectomy. The migration pathway was investigated using newly developed four-color immunohistochemistry and immunofluorescence. Donor TDLs showed rapid transition in gut tissues from which they emerged in mesenteric lymph around 4 h after intravenous injection. Immunohistochemistry showed that donor TDLs predominantly transmigrated across high endothelial venules (HEVs) at the interfollicular area of the Peyer’s patches (PPs), then exited into the LYVE-1+ efferent lymphatics, that were close to the venules. The rapid recirculation depended largely on the local expression of unsulfated sialyl-Lewis X on these venules where putative dendritic cells (DCs) were associated underneath. Recruited naive T cells briefly made contact with resident DCs before exiting to the lymphatics in the steady state. In some transplant settings, however, the T cells retained contact with DCs and were sensitized and differentiated into activated T cells. In conclusion, we directly demonstrated that lymphocyte recirculation within the gut is a very rapid process. The interfollicular area of PPs functions as a strategically central site for rapid immunosurveillance where HEVs, efferent lymphatics and resident DCs converge. PPs can, however, generate alloreactive T cells, leading to exacerbation of graft-versus-host disease or gut allograft rejection.
- Subjects :
- 0301 basic medicine
Pathology
medicine.medical_specialty
alloresponse
Lymphocyte
Immunology
High endothelial venules
T cells
Oligosaccharides
blood-lymph transition
digestive system
Mice
Peyer's Patches
03 medical and health sciences
Cell Line, Tumor
medicine
Animals
Immunology and Allergy
Endothelium
Lymphocytes
dendritic cells
Sialyl Lewis X Antigen
Immunologic Surveillance
Venule
Chemistry
fungi
food and beverages
Peyer's patch
hemic and immune systems
Rats, Inbred Strains
General Medicine
Rats
Intestines
Transplantation
Immunosurveillance
Editor's Choice
030104 developmental biology
medicine.anatomical_structure
Lymphatic system
Blood Circulation
lymphatics
Featured Article of the Month
Lymph
tissues
Subjects
Details
- ISSN :
- 14602377 and 09538178
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- International Immunology
- Accession number :
- edsair.doi.dedup.....1f6610dc95116c36c3c441e2b82734e8