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ACR11 modulates levels of reactive oxygen species and salicylic acid-associated defense response in Arabidopsis
- Source :
- Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
- Publication Year :
- 2018
-
Abstract
- The ACT domain (aspartate kinase, chorismate mutase and TyrA), an allosteric effector binding domain, is commonly found in amino acid metabolic enzymes. In addition to ACT domain-containing enzymes, plants have a novel family of ACT domain repeat (ACR) proteins, which do not contain any recognizable catalytic domain. Arabidopsis has 12 ACR proteins, whose functions are largely unknown. To study the functions of Arabidopsis ACR11, we have characterized two independent T-DNA insertion mutants, acr11-2 and acr11-3. RNA gel-blot analysis revealed that the expression of wild-type ACR11 transcripts was not detectable in the acr11 mutants. Interestingly, a lesion-mimic phenotype occurs in some rosette leaves of the acr11 mutants. In addition, high levels of reactive oxygen species (ROS), salicylic acid (SA), and callose accumulate in the mutant leaves when grown under normal conditions. The expression of several SA marker genes and the key SA biosynthetic gene ISOCHORISMATE SYNTHASE1 is up-regulated in the acr11 mutants. Furthermore, the acr11 mutants are more resistant to the infection of bacterial pathogen Pseudomonas syringae pathovar tomato DC3000. These results suggest that ACR11 may be directly or indirectly involved in the regulation of ROS and SA accumulation, which in turn modulates SA-associated defense responses and disease resistance in Arabidopsis.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Science
Mutant
Arabidopsis
Pseudomonas syringae
01 natural sciences
Article
03 medical and health sciences
chemistry.chemical_compound
Gene Expression Regulation, Plant
Aspartate kinase
Intramolecular Transferases
Disease Resistance
Plant Diseases
Multidisciplinary
biology
Arabidopsis Proteins
RNA Nucleotidyltransferases
biology.organism_classification
Plants, Genetically Modified
Cell biology
Plant Leaves
030104 developmental biology
chemistry
Host-Pathogen Interactions
Mutation
Chorismate mutase
Medicine
ACT domain
Reactive Oxygen Species
Salicylic Acid
Oxidation-Reduction
Salicylic acid
010606 plant biology & botany
Binding domain
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 8
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....1f55a95d448901d3af11f5aa068ba83c